Reivindicaciones
CLAIMS We claim: 1. An isolated chimeric polynucleotide comprising a sequence encoding a mature polyol oxidase protein (POx), said sequence being operably linked to a sequence encoding a secretory signal peptide, said signal peptide being derived from a prokaryotic microorganism. 2. The isolated polynucleotide of Claim 1 , wherein said sequence encoding a mature POx is derived from a Streptomyces sp., an Acidothermus sp. or an Arthrobacter sp. 3. The chimeric polynucleotide of Claim 1 , wherein said chimeric polynucleotide has a sequence selected form SEQ ID NO:35, 37, 38, and 40. 4. The chimeric polynucleotide of Claim 1 , wherein said sequence encoding said secretory signal peptide is selected from the signal sequence encoded by the S. coelicolor gene SCO7637, the signal sequence encoding the S. lividans gene SCO 0624 and the signal sequence encoding the B. subtilis gene P43379. 5. A recombinant expression vector comprising the isolated chimeric polynucleotide of Claim 2. 6. A host cell comprising the recombinant expression vector of Claim 5. 7. A host cell comprising a polynucleotide encoding a POx polypeptide, wherein said Pox polypeptide is selected from SEQ ID NO: 6, 35, 37, 38 and 40. 8. The host cell of Claim 7, wherein said POx has sorbitol oxidase and/or xylitol oxidase activity. 9. The host cell of Claim 7, wherein said polynucleotide is present in the genome of said host cell or in a vector that autonomously replicates in said host cell. 10. The host cell of Claim 7, wherein said host cell is an S. lividans, a B. subtilis or an Acidothermus cellulolyticus host cell. 11. A method for producing a polypeptide having POx activity comprising: (a) transforming a host cell with a recombinant expression vector, said expression vector comprising a polynucleotide sequence encoding a POx polypeptide having a sequence selected from SEQ ID N0:6, 35, 37, 38, and 40; (b) growing said host cell comprising said recombinant expression vector under conditions suitable for the expression of said POx polypeptide; and (c) recovering said POx polypeptide. 12. The method of Claim 11 , wherein said POx activity is sorbitol and/or xylitol activity. 13. The method of Claim 11 , wherein said host cell is selected from Bacillus cells or Streptomyces cells. 14. The method of Claim 11 , wherein said expression of said POx is extracellular. 15. The method of Claim 11 , wherein said expression of said POx is intracellular. 16. A cleaning composition comprising an effective amount of an isolated POx comprising an amino acid sequence that is at least about 70% identical to a POx having a sequence selected from SEQ ID N0:2, 4, 5, 6, 7, 8, 9, 10, 11 , 12, 13, and 14. 17. The cleaning composition of Claim 16, wherein said cleaning composition is a detergent. 18. The cleaning composition of Claim 17, further comprising a bleach activator. 19. The cleaning composition of Claim 16, further comprising at least one additional enzyme. 20. The cleaning composition of Claim 19, wherein said at least one additional enzyme is selected from hemicellulases, peroxidases, proteases, cellulases, xylanases, lipases, phospholipases, esterases, cutinases, pectinases, keratinases, reductases, oxidases, oxidoreductases, perhydrolases, phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, mannanases, [beta]-glucanases, arabinosidases, hyaluronidases, chondroitinases, laccases, and amylasess, or mixtures thereof. 21. The cleaning composition of Claim 19, wherein said at least one additional enzyme is a perhydrolase. 22. The cleaning composition of Claim 19, wherein said at least one additional enzyme is a glucose oxidase. 23. The cleaning composition of Claim 19, wherein said cleaning composition is a bleaching composition. 24. A method of cleaning, comprising the step of contacting a hard surface and/or an article comprising a fabric with the cleaning composition of Claim 16. 25. The method of Claim 24, further comprising the step of rinsing said surface and/or article after contacting said surface or article with said cleaning composition. 26. The method of Claim 24, wherein said surface and/or article comprising a fabric is stained with a substance containing at least one polylol. 27. The method of Claim 26, wherein said polyol is D-sorbitol, D-xylitol, D-mannitol, D- ribitol, myo-inositol, glycerol, 1 ,3,-propanediol or 1 ,2-propanediol. 28. The method of Claim 26, wherein said surface and/or said fabric article is soiled with juice, wine and/or tea.
