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VECTOR (VERSIONS) BASED ON RECOMBINANT DEFECTIVE VIRAL GENOME AND ITS APPLICATION FOR DEVISE OF VACCINE PREPARATIONCM Patents

Índice de la ficha

Updated at
24/07/2026
Numero publicacion
EP.1008652.A1
Fecha publicacion
14/06/2000
Numero solicitud
EP19970907111

En detalle

Resumen

FIELD: genetic engineering, molecular biology. SUBSTANCE: invention relates to vectors comprising defective viral genome that expresses antigen useful for induction of secretory and systemic immune responses and antibody providing protection against infectious agent. The presented defective viral genome is prepared from the parental genome of porcine virus of transmissive gastroenteritis (TGEV) by deleting part of parental genome and retains 3'- and 5'-ends of parental genome with localized recognition signals by viral replicase and at least a promoter. Indicated vectors are useful for design of recombinant system that comprises the indicated expression vector and helper virus. Such system is used for preparing mono- and polyvalent vaccines against infectious agents of animals of different species. Invention proposes also vectors of expression of heterologous genes with high level of safety and capacity of cloning and their construction provides species specificity and tropism that can be controlled easily. EFFECT: valuable properties of vector. 14 cl, 2 tbl, 30 dwg, 5 ex

Reivindicaciones

1. A defective viral genome comprising parental virus genome having viral replicase recognition signals located on ends 3' and 5', further comprising internal deletions, and wherein said defective viral genome depends on a helper virus for its replication. 2. A genome according to claim 1, comprising, additionally, the complete sequence coding for the parental virus replicase. 3. A genome according to the preceding claims, wherein the aforesaid parental virus is a coronavirus. 4. A genome according to the preceding claims, comprising a defective genome of a porcine, canine or feline coronavirus. 5. A genome according to claim 4, comprising a defective genome of the virus of transmissible gastroenteritis of pigs (TGEV). 6. A genome according to any of the preceding claims, wherein the aforesaid helper virus is the parental virus. 7. An expression vector based on a recombinant defective viral genome expressing at least one antigen capable of inducing systemic and secretory immune responses, comprising a defective viral genome according to claims 1 to 6, or its corresponding complementary DNA (cDNA), and, at least, one DNA sequence coding for an antigen capable of conferring systemic and mucosal immunity. 8. A vector according to claim 7, comprising more than one DNA sequence, each one of which codes for a different antigen capable of conferring systemic and mucosal immunity. 9. An expression vector based on a recombinant defective viral genome expressing at least one antibody which confers protection against an infectious agent comprising a defective viral genome according to claims 1 to 6, or its corresponding complementary DNA (cDNA), and, at least, one DNA sequence coding for an antibody that confers protection against one infectious agent. 10. A vector according to claim 9, comprising more than one DNA sequence, each one of which codes for a different antibody that confers protection against an infectious agent. 11. A recombinant system based on recombinant defective viruses expressing, at least, one antigen capable of inducing systemic and mucosal immunity, comprising: a) a recombinant expression vector based on a defective viral genome according to claims 7 or 8, containing, at least, one DNA sequence coding for an antigen capable of conferring systemic and mucosal immunity; and b) a helper virus. 12. A system according to claim 11, wherein the aforesaid expression vector comprises more than one DNA sequence, each one of which codes for a different antigen capable of conferring systemic and mucosal immunity. 13. A system according to claim 11, comprising different expression vectors each one of which contains a different DNA sequence coding for a different antigen capable of conferring systemic and mucosal immunity. 14. A system according to any of claims 11 to 13, wherein the aforesaid helper virus is the parental virus from which the defective viral genome is derived. 15. A system according to claim 14, wherein the aforesaid helper virus provides the functional and structural proteins for the replication and encapsidation of the defective genome. 16. A system according to claim 14, wherein the aforesaid helper virus provides the structural proteins for the encapsidation of the defective genome. 17. A recombinant system based on recombinant defective viruses expressing, at least one antibody that confers protection against an infectious agent, comprising: a) a recombinant expression vector based on a defective viral genome according to claims 9 or 10, containing, at least one DNA sequence coding for an antibody that confers protection against an infectious agent, and b) a helper virus. 18. A system according to claim 17, wherein the aforesaid expression vector comprises more than one DNA sequence, each one of which codes for a different antibody conferring protection against and infectious agent 19. A system according to claim 17, comprising different expression vectors each one of which contains a different DNA sequence coding for an antibody that confers protection against an infectious agent. 20. A system according to any of claims 17 to 19, wherein the aforesaid helper virus is the parental virus from which the defective viral genome is derived. 21. A system according to claim 20, wherein the aforesaid helper virus provides the functional and structural proteins for the replication and encapsidation of the defective genome. 22. A system according to claim 20, wherein the aforesaid helper virus provides the structural proteins for the encapsidation of the defective genome. 23. A vaccine capable of inducing protection in animals against an infectious agent, comprising a suitable quantity of a recombinant system according to any of claims 11 to 22, together with a pharmaceutically acceptable excipient. 24. A vaccine according to claim 23, wherein the aforesaid recombinant system comprises an expression vector containing a DNA sequence coding for an antigen capable of conferring systemic and mucosal immunity. 25. A vaccine according to claim 23, wherein the aforesaid recombinant system comprises an expression vector containing more than one DNA sequence, each one of which codes for a different antigen capable of conferring systemic and mucosal immunity. 26. A vaccine according to claim 23, wherein the aforesaid recombinant system comprises different expression vectors each one of which contains at least one different DNA sequence coding for a different antigen capable of conferring systemic and mucosal immunity. 27. A vaccine according to claim 23, suitable for the conferring of immunity against porcine infectious agents, wherein the recombinant system comprises at least one expression vector containing at least one DNA sequence coding for a porcine pathogen antigen. 28. A vaccine according to claim 23, suitable for the conferring of immunity against porcine infectious agents, wherein the recombinant system comprises one expression vector containing more than one DNA sequence, each one of which codes for a different porcine pathogen antigen. 29. A vaccine according to claim 23, suitable for the conferring of immunity against porcine infectious agents, wherein the recombinant system comprises different expression vectors each one of which contains at least one DNA sequence coding for a porcine pathogen antigen. 30. A vaccine according to claims 27 to 29, wherein the aforesaid porcine pathogen is selected from a group consisting essentially of: Actinobacillus suis, Actinobacillus pleuropneumoniae, Haemophilus parasuis, Porcine parvovirus, Leptospira, Escherichia coli, Erysipelothrix rhusiopathiae, Pasteurella multocida, Bordetella bronchiseptica, Clostridium sp., Serpulina hyodysenteriae, Mycoplasma hyopneumoniae, porcine epidemic diarrhea virus (PEDV), porcine respiratory coronavirus, rotavirus, or against the pathogens causative of porcine reproductive and respiratory syndrome, Aujeszky's disease (pseudorabies), swine influenza or transmissible gastroenteritis and the etiologic agents of atrophic rhinitis and proliferative ileitis. 31. A vaccine according to claim 23, suitable for the conferring of immunity against canine infectious agents, wherein the recombinant system comprises at least one expression vector containing at least one DNA sequence coding for a canine pathogen antigen. 32. A vaccine according to claim 23, suitable for the conferring of immunity against canine infectious agents, wherein the recombinant system comprises an expression vector containing more than one DNA sequence, each one of which codes for a different canine pathogen antigen. 33. A vaccine according to claim 23, suitable for the conferring of immunity against canine infectious agents, wherein the recombinant system comprises different expression vectors each one of which contains at least one DNA sequence coding for a canine pathogen antigen. 34. A vaccine according to claims 31 to 33, wherein the aforesaid canine pathogen is selected from a group constituted essentially of: canine herpesviruses, canine adenovirus types 1 and 2, canine parvovirus types 1 and 2, canine reovirus, canine rotavirus, canine coronavirus, canine parainfluenza virus, canine influenza virus, distemper virus, rabies virus, retovirus and canine calicivirus. 35. A vaccine according to claim 23, suitable for the conferring of immunity against feline infectious agents, wherein the recombinant system comprises at least one expression vector containing at least one DNA sequence coding for a feline pathogen antigen. 36. A vaccine according to claim 23, suitable for the conferring of immunity against feline infectious agents, wherein the recombinant system comprises one expression vector containing more than one DNA sequence, each one of which codes for a feline pathogen antigen. 37. A vaccine according to claim 23, suitable for the conferring of immunity against feline infectious agents, wherein the recombinant system comprises different expression vectors each one of which contains at least one DNA sequence coding for one feline pathogen antigen. 38. A vaccine according to claims 35 to 37, wherein the aforesaid feline pathogen is selected from a group constituted essentially of: feline calicivirus, feline immunodeficiency virus, feline herpesviruses, feline panleukopenia virus, feline reovirus, feline rotavirus, feline coronavirus, feline infectious peritonitis virus, rabies virus, feline Chlamydia psittaci and feline leukemia virus. 39. A vaccine according to claim 23, wherein the aforesaid recombinant system comprises an expression vector containing a DNA sequence coding for an antibody that confers protection against an infectious agent. 40. A vaccine according to claim 23, wherein the said recombinant system comprises an expression vector containing more than one DNA sequence, each one of which codes for an antibody conferring protection against an infectious agent. 41. A vaccine according to claim 23, wherein the said recombinant system comprises different expression vectors each one of which contains at least a different DNA sequence coding for an antigen that confers protection against an infectious agent 42. A vaccine according to claim 23, suitable for the conferring of immunity against a porcine infectious agent, wherein the recombinant system comprises at least one expression vector containing at least one DNA sequence coding for an antibody that confers protection against the aforesaid porcine infectious agent. 43. A vaccine according to claim 42, wherein the recombinant system comprises at least one expression vector containing at least one DNA sequence coding for the monoclonal antibody identified as 6A.C3 capable of neutralizing the virus of transmissible gastroenteritis of pigs (TGEV). 44. A vaccine according to claim 23, wherein the helper virus of the recombinant system comprises a coronavirus. 45. A vaccine according to claim 44, wherein the aforesaid coronavirus is selected from the group composed of porcine, canine and feline coronaviruses.

Etiquetas

Inventores
Sanchez Luis EnjuanesDuran Juan PlanaVillanueva Sara AlonsoJarreio M Luisa BallesterosCastrillon Joaquin CastillaMartinez Jose Manuel GonzalezParmesan Ander IzetaZunzunegui Ana MendezSaenz Maria MuntionPenzes ZoltanMorgado Jose Manuel SanchezSanchez Carlos Miguel SanchezPicazo Cristian SmerdouGurpegui Isabel SolaJarreno Ma Luisa BallesterosLuis Enjuanes SanchezJuan Plana DuranSara Alonso VillanuevaMð Luisa Ballesteros JarreioJoaquin Castilla CastrillonJose Manuel Gonzalez MartinezAnder Izeta ParmesanAna Mendez ZunzuneguiMaria Muntion SaenzZoltan PenzesJose Manuel Sanchez MorgadoCarlos Miguel Sanchez SanchezCristian Smerdou PicazoIsabel Sola GurpeguiMa Luisa Ballesteros JarrenoJarreno Maria Luisa BallesteroMaria Luisa Ballesteros JarrenoMuntion Saenz MariaSmerdou Picazo CristianSanchez Sanchez Carlos MiguelBallesteros Jarreno LuisaAlonso Villanueva SaraCastilla Castrillon JoaquinSola Gurpegui IsabelIzeta Parmesan AnderPlana Duran JuanGonzalez Martinez Jose ManuelMendez Zunzunegui AnaSanchez Morgado Jose ManuelEnjuanes Sanchez LuisSanchez L EngjuanesDuran J PlanaVillanueva S AlonsoVillanueva L Engjuanes SanchezL. Engjuanes SanchezJ. Plana DuranS. Alonso VillanuevaL. Engjuanes Sanchez,J. Plana Duran,S. Alonso VillanuevaBallesteros Jarreno M LuisaGonzales Martinez Jose ManuelMendez Zuzunegui AnaPenzes Zoltan Ph DGurpegui Isobel SolaBallesteros Jareno Maria LuisaSanchez Sanchez Carlos M엔후아네스 산체즈 루이스플라나 듀란 후안알론소 빌라뉴에바 사라발레스테로스 하레뇨 마 루이사카스틸라 카스트릴론 호아퀸곤잘레즈 마르티네즈 호세 마뉴엘이제타 파르메산 안데르멘데즈 준주네구이 안나문티온 사엔즈 마리아펜제스 졸탄산체즈 모르가도 호세 마뉴엘산체즈 산체즈 카를로스 미구엘스메르도우 피카조 크리스티안솔라 구르페구이 이사벨Ehnkhuanes Sanches LuisPlana Duran KhuanAlonso Vil Januehva SaraBal Esteros Kharren O Marija LKastil Ja Kastril On KhoakinGonsales Martines Khose ManuehIseta Parmesan AnderMendes Sunsunegi AnaMuntion Saens MarijaPenses Sol TanSanches Morgado Khose ManuehlSanches Sanches Karlos MigelSmerdou Pikaso KristianSola Gurpegi IsabelBal'Esteros Kharren'O Marija LuisaGonsales Martines Khose Manuehl'Jarreno M Luisa BallesterMartinez Jose Manuel GonzalesPicazo Cristina SmerdouJarreno M?AposLuisa BallesterosSanchez Luis eDuran Juan PVillanueva Sara AJarreno M Luisa BCastrillon Joaquin CMartinez Jose M GParmesan Ander IZunzunegui Ana MSaenz Maria MMorgado Jose M SSanchez Carlos M SPicazo Cristina SGurpegui Isabel SJarreno Maluisa BallesterosBallesteros Jarreno Ma Luisa
Solicitantes
Wyeth Farma SaIberica CyanamidCyanamid Iberica, SASianamid Iberika S ASanchez Luis EnjuanesDuran Juan PlanaVillanueva Sara AlonsoJarreno M?AposLuisa BallesterosCastrillon Joaquin CastillaMartinez Jose Manuel GonzalesParmesan Ander IzetaZunzunegui Ana MendezSaenz Maria MuntionPenzes ZoltanMorgado Jose Manuel SanchezSanchez Carlos Miguel SanchezPicazo Cristina SmerdouGurpegui Isabel SolaEnjuanes Sanchez LuisPlana Duran JuanAlonso Villanueva SaraBallesteros Jarreno Ma LuisaCastilla Castrillon JoaquinGonzalez Martinez Jose ManuelIzeta Parmesan AnderMendez Zunzunegui AnaMuntion Saenz MariaSanchez Morgado Jose ManuelSanchez Sanchez Carlos MiguelSmerdou Picazo CristianSola Gurpegui Isabel
Clasificacion ipc
A61K 35/ 76 A IA61K 39/ 12 A IA61K 39/ 215 A IA61K 39/ 225 A IA61K 39/ 395 A IA61K 48/ 00 A IA61P 31/ 00 A IA61P 31/ 12 A IA61P 31/ 14 A IC07K 14/ 17 A IC07K 16/ 08 A IC07K 16/ 10 A IC12N 15/ 09 A IC12N 15/ 50 A IC12N 15/ 86 A IC12N 7/ 01 A IC12N 15/ 00 A IC12P 21/ 06 A I
Clasificacion cpc
4B024/AA014B024/AA104B024/BA314B024/BA324B024/BA514B024/CA044B024/CA124B024/DA204B024/EA024B024/EA044C084/AA134C084/MA134C084/MA524C084/MA594C084/MA654C084/ZB0514C084/ZB0524C084/ZB0924C084/ZC6114C084/ZC6124C085/AA034C085/AA044C085/AA134C085/BA014C085/BA124C085/BA154C085/BA184C085/BA214C085/BA454C085/BA464C085/BA484C085/BA524C085/BA554C085/BA564C085/BA574C085/BA604C085/BA644C085/BA664C085/BA674C085/BA714C085/BA734C085/BA754C085/BA774C085/BA784C085/CC214C085/CC224C085/CC404C085/DD624C085/EE014C085/GG034C085/GG044C085/GG054C085/GG064C085/GG084C085/GG104C087/AA014C087/AA024C087/BB374C087/BB574C087/BB654C087/BC344C087/BC444C087/BC684C087/BC814C087/BC824C087/BC834C087/BC904C087/CA054C087/CA124C087/CA164C087/CA174C087/CA324C087/MA024C087/MA134C087/MA164C087/MA234C087/MA244C087/MA444C087/MA524C087/MA564C087/MA594C087/MA654C087/MA704C087/NA054C087/NA064C087/NA074C087/ZC614H045/AA114H045/AA204H045/AA304H045/CA014H045/DA754H045/DA864H045/EA314H045/FA724H045/FA74A61K35/76A61K39/215A61K39/225A61K39/395&DA61K39/395&SA61K48/00A61P31/12&171A61P31/14C07K16/08C12N15/00&A424/93.21435/457435/320.1435/69.1424/221.1435/236
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