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USE OF BETA-D-GALACTOPYRANOSYL-D-XYLOSES FOR THE PREPARATION OF COMPOSITIONS AND SOLUTIONS INTENDED TO THE EVALUATION OF INTESTINAL LACTASE, AND PRODUCTION PROCESS OF THE SAID COMPDS.CM Patents

1. Use of β-D-galactopyranosyl-D-xylose having two hydroxyl groups each of which is adjacent respectively to the glycosidic bond in its molecule and selected from a group comprising 2-O-β-D-galactopyranosyl-D-xyloses, 3-O-β-D-galactopyranosyl-D-xyloses and mixtures of the same for the preparation of compositions and solutions intended to the evaluation of intestinal lactase. 2. Use of β-D-galactopyranosyl-D-xylose having two hydroxyl groups each of which is adjacent respectively to the glycosidic bond in its molecule and selected from a group comprising 2-O-β-D-galactopyranosyl-D-xyloses, 3-O-β-D-galactopyranosyl-D-xyloses and mixtures of the same for the preparation of compositions comprising 4-O-β-D-galactopyranosyl-D-xyloses intended to the evaluation of intestinal lactase. 3. Composition for the evaluation of intestinal lactase comprising β-D-galactopyranosyl-D-xylose as an active compound, characterized in that β-D-galactopyranosyl-D-xylose comprises β-D-galactopyranosyl-D-xylose having two hydroxyl groups each of which is adjacent respectively to the glycosidic bond in its molecule and selected from a group comprising 2-O-β-D-galactopyranosyl-D-xyloses, 3-O-β-D-galactopyranosyl-D-xyloses and mixtures of the same and also pharmaceutically acceptable quantities of at least one additive. 4. Composition for the evaluation of intestinal lactase comprising 4-O-β-D-galactopyranosyl-D-xylose, characterized in that further comprises 2-O-β-D-galactopyranosyl-D-xyloses, 3-O-β-D-galactopyranosyl-D-xyloses and mixtures of the same and also pharmaceutically acceptable quantities of at least one additive. 5. Composition according to claim 3 or 4, characterized in that comprises an additive selected from among stabilizers, protectors, taste enhances, lactose, gels, fluxants and conservatives. 6. Composition according to claim 3 or 4, characterized in that the solutions are aqueous. 7. Composition according to claim 3 or 4, characterized in that the solutions are saline. 8. Composition according to claim 3 or 4, characterized in that comprises an additive selected form among stabilizers, protectors, taste enhances, lactose, and conservatives.

Datos clave

CategoríaInvención

Índice de la ficha

Updated at
05/11/2026
Numero publicacion
EP.0819764.A1
Fecha publicacion
21/01/1998
Anio publicacion
1998

En detalle

Reivindicaciones

1. Use of β-D-galactopyranosyl-D-xyloses for the preparation of compositions and solutions intended to the evaluation of intestinal lactase, characterized in that - the β-D-galactopyranosyl-D-xyloses present several hydroxyl groups adjacent respectively to the glycosidic bond in its molecule. 2. Use according to claim 1, characterized in that the β-D-galactopyranosyl-D-xyloses are selected from among the 2-O-β-D-galactopyranosyl-D-xylose, 3-O- β-D-galactopyranosyl-D-xylose and mixtures of the same. 3. Use according to claim 1 or 2, characterized in that the compositions comprise pharmaceutically acceptable quantites of at least one additive selected from among stabilizers, protectors, taste enhances, lactose , gels, fluxants and conservatives. 4. Use according to any of the claims 1 or 2, characterized in that the solutions are aqueous. 5. Use according to claim 4, characterized in that the solutions are saline. 6. Use according to any of the claims 1, 2, 4 or 5, characterized in that the solutions comprise pharmaceutically acceptable quantities of at least one additive selected from among stabilizers, protectors, taste enhances, lactose and conservatives. 7. Enzymatic procedure for the preparation of β-D-galactopyranosyl-D-xyloses, which comprises at least one β-D-galactopyranosyl-D-xylose which presents several hydroxyl groups adjacent respectively to the glycosidic bond in its molecule, characterized in that it comprises: (a) reactivating a D-xylose and a β-D-galactopyranoside substrate in the presence of a β-galactosidase enzyme, to a concentration of D-xylose / β-D-galactopyranoside substrate of 2-20:1, in an aqueous medium buffered to a pH between 5,0 and 9,0 and at a temperature between 4 and 37°C ; (b) deactivating the β-galactosidase enzyme by heating at 100°C when reaching the maximum yield of formation of disaccharides, based on thin layer chromatography; (c) isolating the disaccharides formed through filtration in a packed column with a thinner selected between water and mixtures of water and alcohol. 8. Procedure according to claim 7, characterized in that the β-D-galactpyranoside substrate is Ó-nitrophenyl β-D-galactpyranoside. 9. Procedure according to claim 7, characterized in that the β-D-galactopyrandoside is substrate lactose. 10. Procedure according to claim 7, characterized in that the β-galactosidase enzyme is β-D-galactosidase of E. Coli . 11. procedure according to claim 7, characterized in that it is conducted in the presence of at least one cosolvent, miscible with water. 12. Procedure according to claim 11, characterized in that the cosolvent is selected from among at least acetonitrile, dimethyl formamide, dimethyl sulfoxide and mixture of the same. 13. Procedure according to claim 7, characterized in that the packed columns are packed with one of Sephadex G-10 and Biogel P-2. 14. Procedure according to claim 7, characterized in that the packed columns are packed with active carbon. 15. Procedure according to claim 7 or 10, characterized in that the formed disaccharides are a mixture of 2-O-β-D-galactopyranosyl-D-xylose, 3-O-β-D-galactopyranosyl-D-xylose and 4-O-β-D-galactopyranosyl-D-xylose. 16. Procedure of claim 10, characterized in that from the formed disaccharides, at least one of the 2-O-β-D-galactopyranosyl-D-xylose, 3-O-β-D-galactopyranosyl-D-xylose, is isolated.

Etiquetas

Inventores
Aragon Reyes, Juan JoseAragon Reyes Juan JoseCanada Vicinay Francisco JavieCanada Vicinay, Francisco JavierFernandez-Mayoralas Alvarez AlFernandez-Mayoralas Alvarez, AlfonsoLopez Alvarez RosaLopez Alvarez, RosaMartin Lomas, ManuelMartin Lomas ManuelVillanueva Torregroza DanielVillanueva Torregroza, DanielJuan Jose Aragon ReyesReyes Juan Jose AragonFrancisco Javier Canada VicinayVicinay Francisco Javier CanadAlvarez Alfonso Fernandez-MayoAlfonso Fernandez-Mayoralas AlvarezRosa Lopez AlvarezAlvarez Rosa LopezLomas Manuel MartinManuel Martin LomasDaniel Villanueva TorregrozaTorregroza Daniel VillanuevaVicinay Francisco Javier CanadaAlvarez Afonso Fernandez MayoralasMayoralas Alvarez Afonso FernaMatin Lomas ManuelLomas Manuel MatinReyes J J AragonJ. J. Aragon ReyesF. J. Canada VicinayVicinay F J CanadaA. Fernandez-Mayoralas AlrarezAlrarez A Fernandez-MayoralasAragon Reyes Juan JoséCanada Vicinay Francisco JavierFernandez-Mayoralas Alvarez AlfonsoАрагон Рейес, Хуан ХосеКанада Висинай, Франсиско ХавьерФернандес-Майоралас Альварес, АльфонсоЛопес Альварес, РозаМартин Ломас, МануэльВиллануэва Торрегроса, ДаниэльCanada Vicinay Francisco J.Canada Vicinay Francisco JFernandez-Mayorales Alvarez AlFernandez-Mayorales Alvarez AlfonsoVinllanueva Torregroza DanielAragon Reyes, Juan JoséFernandez-Mayoralas Alvarez A아라곤 레예스, 후안 호세까나다 비시나이, 프란시스꼬 하비에르페르난데스 마요랄라스 알바레스, 알폰소로뻬스 알바레스, 로사마르띤 로마스, 마누엘비야누에바 또레그로사, 다니엘Vicina Francisco Javier CanadaFrancisco Javier Canada VicinaAlvarez Alfonso Fernandez MayoAlfonso Fernandez Mayoralas AlvarezReyes, Juan Jose AragonVicinay, Francisco Javier CanadaAlvares, Alfonso Fernandez-MayoralasAlvares Alfonso Fernandez-MayoAlvares Rosa LopezAlvares, Rosa LopezLomas, Manuel MartinTorregroza, Daniel VillanuevaAlvarez, Alfonso Fernandez-MayoralasAlvarez, Rosa LopezAragon Reyes J. J.Aragon Reyes J JCanada Vicinay F JCanada Vicinay F. J.Fernandez-Mayoralas Alvarez A.Lopez Alvarez R.Lopez Alvarez RMartin Lomas M.Martin Lomas MVillanueva Torregroza DVillanueva Torregroza D.Aragon ReyesJuan JoseCanada VicinayFrancisco JavierFernandez-Mayoralas AlvarezAlfonsoLopez AlvarezRosaMartin LomasManuelVillanueva TorregrozaDaniel
Solicitantes
Consejo Superior de Investigaciones CientíficasUniv AutonomaConsejo Superior Investigactione ScientificasConsejo Superior Investigatione ScientificasConsejo Superior Invest e ScieUniversidad Autónoma de MadridУниверсидад Аутонома
Clasificacion ipc
C07H 3/ 04 A IC12P 19/ 14 A IC12Q 1/ 34 A IC12Q 1/ 40 A I
Clasificacion cpc
4B063/QA014B063/QA194B063/QQ034B063/QQ354B063/QQ684B063/QR434B063/QR724B063/QS024B063/QX014B064/AF034B064/CA024B064/CA214B064/CD094B064/CE074B064/DA134C057/AA194C057/BB014C057/BB03C07H3/04C12P19/14&ZC12Q1/344B063/QQ024B063/QR154B063/QR234B063/QR414B063/QR444B063/QR514B063/QR534B063/QR574B063/QS174B064/CC034B064/CC064B064/CC074B064/CC084B064/CC134B064/CD114B064/CD124C057/AA034C057/AA30C12Q1/40435/100435/14435/18435/97435/99
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