[0001] This invention pertains to the chemical field, in particular, to the field of pharmaceutical or galenic formulation, more particularly, it relates to methods to formulate homogenous dispersions and emulsions of bioactive substances in oily matrixes.
1. A method to incorporate bioactive compounds in a lipid phase using supercritical carbon dioxide technology, wherein the method comprises: a) Loading a high-pressure vessel with a lipid phase, optionally including a surfactant; b) Pumping CO<2> into the pressure vessel until supercritical (SC) conditions are reached; c) A dissolution comprising a solvent and a bioactive solute or a mixture of bioactive solutes, is pumped into the high-pressure vessel through a nozzle; together with the SCCO<2> flow set in step b); and d) Collecting the lipid dispersion or emulsion formed in the lipid phase of step a). 2. The method of claim 1, wherein the liquid of step c) is a dissolution comprising a solvent, wherein the solvent is an organic solvent which can be dissolved in SCCO2 in a concentration of at least 2% w/w, and a bioactive solute or a mixture of bioactive solutes. 3. The method of claim 1, wherein the liquid of step c) is a dissolution comprising water or water-solvent mixtures, and a bioactive solute or a mixture of bioactive solutes. 4. The method of claim 2, wherein the liquid of step is selected from the list consisting of olive oil, flaxseed oil, sunflower oil, fish oils, essential oils, argan oil, triglycerides and mixtures thereof; and the solvent of step c) is selected from the list consisting of C1-C4 alcohols, preferable methanol and ethanol, isopropanol, methyl acetate, ethyl acetate, ethyl lactate, DMSO, acetone, and mixtures thereof. 5. The method of claim 3, wherein the liquid of step a) is selected from the list consisting of olive oil, flaxseed oil, sunflower oil, fish oils, essential oils, argan oil, triglycerides and mixtures thereof; and the solvent of step c) is selected from the list consisting of water or water-ethanol mixtures. 6. The method of any of claims 1, 2 or 4, wherein the bioactive solutes of step c) are selected from the list consisting of quercetin, rosemary bioactive compounds (rosmarinic acid, carnosic acid, carnosol), licorice bioactive compounds (liquiritin, liquiritigenin, glycyrrhizic acid, isoliquiritigenin, glabridin) and mixtures thereof. 7. The method of any of claims 1, 3 or 5, wherein the bioactive solutes of step c) are selected from the list consisting of saponins, rosmarinic acid, liquiritin and mixtures thereof. 8. The method according to any of claims 1, 2, 4 and 6, wherein if the solvent of the solution is soluble in SCCO2, the liquid of step c) comprises the bioactive molecule or mixture of bioactive substances and the solvent in a weight ratio of 1:15 to 1:300, preferably of 1:30 to 1:160, and more preferably 1:50 to 1:110. 9. The method according to any of claims 1, 2, 4, 6 and 8, wherein if the solvent of the solution is soluble in SCCO2, the liquid phase of step c) and the SCCO2 are admixed in a weight ratio within the range of 1:4 to 1:120, preferably of 1:7 to 1:60, and more preferably 1:20 to 1:40. 10. The method according to any of claims 1, 2, 4, 6, 8 and 9, wherein the collected liquid phase of step c) has solid bioactive particles encapsulated of size of 0.1-5.0 µm, more preferably 0.4-1.3 µm. 11. The method according to any of claims 1, 3, 5, 7, wherein the collected liquid phase of step c) has droplets encapsulated with a volume weighted average size of 5 to 200 µm, more preferably 45 to 110 µm. 12. A solid-liquid dispersion or a water-lipid emulsion in a lipophilic liquid obtainable by the method according to any of claims 1 to 11. 13. A lipid substance with solid bioactive particles homogeneously dispersed in a lipid phase, characterized by a lipid phase selected from the list consisting of olive oil, flaxseed oil, sunflower oil, fish oils, argan oil, triglycerides and mixtures thereof, with solid bioactive particles homogeneously dispersed in the lipid phase selected from the list consisting of quercetin, rosemary bioactive compounds (rosmarinic acid, carnosic acid, carnosol), licorice bioactive compounds (liquiritin, liquiritigenin, glycyrrhizic acid, isoliquiritigenin, glabridin) and mixtures thereof, characterized by a normal particle size distribution with mean size of 0.4-1.3 µm, and a mass concentration of bioactives of 0.2-3.0% (2-30 mg/g). 14. An oil-water emulsion in a weight ratio within the range of 10:90 to 30:70, more preferably 20:80, in which the water phase comprises bioactive solutes selected from the list consisting of any type of saponins, rosmarinic acid and liquiritin and mixtures thereof, characterized by a drop size in the range 45-110 µm, and a mass concentration ofbioactives of 0.2-3.0% (2-30 mg/g); and the lipid phase is selected from the list consisting of olive oil, flaxseed oil, sunflower oil, fish oils, argan oil, triglycerides and mixtures thereof,. 15. A solid-liquid dispersion or the water-lipid emulsion of claim 12, a lipid substance with solid bioactive particles of claim 13, or an oil-water emulsion of claim 14, for use in therapy. 16. A non-therapeutic use of the solid-liquid dispersion or the water-lipid emulsion of claim 12, the lipid substance with solid bioactive particles of claim 13 or the oil-water emulsion of claim 14, for applications in the field of cosmetics. 17. A pharmaceutical composition comprising the solid-liquid dispersion or the water-lipid emulsion of claim 12, the lipid substance with solid bioactive particles of claim 13, or the oil-water emulsion of claim 14. 18. A cosmetic composition comprising the solid-liquid dispersion or the water-lipid emulsion of claim 12, the lipid substance with solid bioactive particles of claim 13, or the oil-water emulsion of claim 14. 19. A nutraceutical composition or food drug composition comprising the solid-liquid dispersion or the water-lipid emulsion of claim 12, the lipid substance with solid bioactive particles of claim 13, or the oil-water emulsion of claim 14.