Resumen
Procedure of immobilization of a glutamate dehydrogenase. This application describes the preparation of a stabilized immobilized thermus spp. Glutamate dehydrogenase (gdh) derivative by multi-point covalent binding. The soluble enzyme is already very stable from ph 6 to ph 10, even at 60º c, it recognizes nad, nadh, nadp, and nadph. Its immobilization in glyoxal supports allows the stabilization of both its multimeric structure and the rigidity of each monomer, further increasing its thermoresistance, and extending the range of use to ph below 5 (where the soluble enzyme is inactivated by dissociation). The immobilized derivative is resistant to solvents, high temperature, drastic ph, etc. This immobilized derivative of the gdh, therefore, would have excellent possibilities of use as a cofactor regenerative enzyme in enzymatic reduction-oxidation processes or as a biosensor. (Machine-translation by Google Translate, not legally binding)