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[0000] The present invention relates to a feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about pH3.5.

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CLAIMS 1. A feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about pH3.5. 2. The feed supplement according to claim 1 wherein said lipolytic enzyme comprises a polypeptide having the sequence of SEQ ID NO: 7 or SEQ ID NO: 8 or a polypeptide having at least 70% identity thereto; or a polypeptide which is produced by expression of a nucleotide sequence comprising the sequence of SEQ ID NO: 9 or 10; or a sequence which differs from SEQ ID NO:9 or 10 due to the degeneracy of the genetic code; or a sequence which has at least 70% identity to SEQ ID NO: 9 or 10. 3. The feed supplement according to claim 1 or claim 2 wherein the lipolytic enzyme is produced in a Trichoderma reesei cell, 4. The feed supplement according to any one of claims 1 to 3, wherein the phytase comprises an E. coli phytase having a the sequence of SEQ ID NO: 13 and/or a Buttiauxella phytase having a the sequence of any one of SEQ ID NOs 1-6 or a polypeptide having at least 70% identity thereto or a polypeptide produced by expression of a nucleotide sequence comprising the sequence of SEQ ID NO: 11; or nucleotides 253 to 1483 of SEQ ID NO:14 or a sequence which differs from SEQ ID NO: 11 or nucleotides 253 to 1483 of SEQ ID NO: 14 due to the degeneracy of the genetic code; or a sequence which has at least 70% identity to SEQ ID NO: 11 or nucleotides 253 to 1483 of SEQ ID NO:14. 5. The feed supplement according to any preceding claim, wherein said phytase has phytase activity at a pH in the range of about pH 2.5 to about pH 5.5. 6. A feed supplement comprising: i) a lipolytic enzyme which is a lipolytic enzyme characterised by at least one of the following characteristics:- a) a lipolytic enzyme that has lipase activity at a pH in the range of about pH 1.5 to about pH 3.5 when measured by the lipase assay disclosed herein; b) a lipolytic enzyme which comprises a polypeptide comprising the amino acid sequence as shown in SEQ ID NO: 7 or 8 or a polypeptide having at least 70% identity thereto; c) a lipolytic enzyme which is produced by expression of a nucleotide sequence comprising the sequence of SEQ ID NO: 9 or 10; or a sequence which differs from SEQ ID NO:9 or 10 due to the degeneracy of the genetic code; or a sequence which has at least 70%identity to SEQ ID NO: 9 or 10; and ii) a phytase which characterised by at least one of the following chara cteri sties :- d) a phytase that has phytase activity when measured at a pH in the range of about pH 2.5 to about pH 5.5; e) a phytase which comprises a polypeptide comprising the amino acid sequence as shown in any one of SEQ ID NOs: 1-6 or SEQ ID NO: 13 or a polypeptide having at least 70% identity thereto; f) a phytase which is produced by expression of a nucleotide sequence comprising the sequence of SEQ ID NO: 1 1 or nucleotides 253 to 1483 of SEQ ID N0:14or a sequence which differs from SEQ ID NO: 11 or nucleotides 253 to 1483 of SEQ !D NO:14due to the degeneracy of the genetic code; or a sequence which has at least 70% identity to SEQ ID NO: 11 or nucleotides 253 to 1483 of SEQ ID NO:14 7. The feed supplement according to claim 5 or claim 6, wherein said phytase also has phytase activity at a pH in the range of about pH 5.0 to about pH 5.5. 8. The feed supplement according to any preceding claim, wherein the phytase has a specific activity level of at least 100 FTU/mg 9. The feed supplement according to any preceding, wherein the lipolytic enzyme has a specific activity level of at least 100 LlPU/mg 10. The feed supplement according to any preceding claim, wherein the ratio of lipolytic enzyme to phytase is in the range of about 1 :3 to about 12:1. 11. The feed supplement according to any preceding claim further comprising at least one physiologically acceptable carrier. 12. The feed supplement according to claim 11, wherein the physiologically acceptable carrier is selected from at least one of maltodextrin, limestone (calcium carbonate), cyclodextrin, wheat or a wheat component, sucrose, starch, anti-foam, Na<2>SO<4>, Talc, and PVA and mixtures thereof. 13. The feed supplement according to any preceding claim, wherein the feed supplement comprises at least one further enzyme. 14. The feed supplement according to claim 13, wherein the at least one further feed enzyme is selected from the group consisting of those involved in starch metabolism, fibre degradation, lipid metabolism, proteins or enzymes involved in glycogen metabolism, acetyl esterases, aminopeptidases, amylases, arabinases, arabinofuranosidases, carboxypeptidases, catalases, cellulases, chitinases, chymosin, cutinase, deoxyribonucleases, epimerases, esterases, -galactosidases, -gϊucanases, glucan lysases, endo-glucanases, glucoamylases, glucose oxidases, -glucosidases, including β glucosidase, glucuronidases, hemicelluiases, hexose oxidases, hydrolases, invertases, isomerases, laccases, lyases, mannosidases, oxidases, oxidoreductases, pectate lyases, pectin acetyl esterases, pectin depolymerases, pectin methyl esterases, pectinoiytic enzymes, peroxidases, phenoloxidases, polygalacturonases, proteases, rhamno-galacturonases, ribonucleases, thaumatin, transferases, transport proteins, transglutaminases, xylanases, hexose oxidase (D-hexose: 02-oxidoreductase, EC 1.1.3.5) β-glucanase, α-amylase, pectinase, cellobiohydrolase, acid phosphatases and/or others or combinations thereof. 15. The feed supplement according to claim 13 or 14 comprising at least one xylanase and/or at least one amylase 16. The feed supplement according to any preceding claim comprising at least 1% by weight, least 5%; at least 10%; at least 15%; at least 20%; or at least 25% by weight of lipolytic enzyme and phytase. 17. A feedstuff comprising the feed supplement or at least one lipolytic enzyme and at least one phytase according to any preceding claims. 18. The feedstuff according to claim 16, comprising one or more feed materials selected from the group comprising a) cereals, such as small grains (e.g., wheat, barley, rye, oats and combinations thereof) and/or large grains such as maize or sorghum; b) by products from cereals, such as corn gluten meal, Distillers Dried Grain Solubles (DDGS), wheat bran, wheat middlings, wheat shorts, rice bran, rice hulls, oat hulls, palm kernel, and citrus pulp; c) protein obtained from sources such as soya, sunflower, peanut, lupin, peas, fava beans, cotton, canola, fish meal, dried plasma protein, meat and bone meal, potato protein, whey, copra, sesame; d) oils and fats obtained from vegetable and animal sources; e) minerals and vitamins. 19. The feedstuff according to claim 17 comprising at least one low fibre feed material, selected from the group consisting of corn, wheat, an animal by-product meal or soybean and/or at least one by-product of the at least one low fibre feed material to provide a low fibre foodstuff. 20. The feedstuff according to any one of claims 16 to 18 comprising a feedstuff which contains at least 30%, at least 40%, at least 50% or at least 60% by weight of corn and soybean meal or corn and full fat soy. 21. The feedstuff according to any one of claims 16 to 19 comprising phytase at a level of about 200FTU/kg to about 15,000 FTU/kg (e.g. 300 to 10,000 FTU/kg, 400 - 7,500 FTU/kg and also 500 - 5000 FTU/kg). 22. The feedstuff according to any one of claims 16 to 20 comprising lipolytic enzyme at a level of about 125 LIPU/kg to about 45,000 LIPU/kg (e.g. 125 to 30,000 LIPU/kg, 1000 - 20000 LIPU/kg and also 3000 - 10000 UPU/kg). 23. The feedstuff according to any one of claims 16 to 21 comprising at least 0.0001%, at least 0.0005%; at least 0.0010%; at least 0.0020%; at least 0.0025%; at least 0.0050%; at least 0.0100%; at least 0.020%; at least 0.100% at least 0.200%; at least 0.250%; at least 0.500% by weight of the feed supplement. 24. The feedstuff according to any one of claims 16 to 22 for consumption by a monogastric animal, selected from the group consisting of poultry, swine, domestic pets or fish. 25. The feedstuff according to any one of claims 16 to 23 comprising at least one further additive. 26. A method of making a feedstuff according to any one of claims 16 to 24 comprising adding to a feed material a feed supplement according to any one of claims 1 to 15, 27. A method of making a feed supplement according to any one of claims 1 to 15 comprising mixing at least one lipolytic enzyme and at least one phytase. 28. The method of claim 26, further comprising the addition of at least one physiologically acceptable carrier selected from at least one of maltodextrin, limestone (calcium carbonate), cyciodextrin, wheat or a wheat component, sucrose, starch, anti-foam, Na<2>SO<4>, Talc, PVA and mixtures thereof. 29. The method according to any one of claims 25 to 27 comprising the additional step of homogenising the feed supplement. 30. The method according to any one of claims 25 to 28 comprising the additional step of pelleting the feed supplement. 31. A method for increasing the availability of at least one dietary nutrient and/or increasing the apparent metabolisable energy (AME) from a feed material comprising adding to feed material a feed supplement according to any one of claims 1 to 15. 32. A method of increasing the growth rate of an animal comprising feeding the animal an effective amount of a feedstuff according to any one of claims 16 to 24. 33. Use of at least one phytase and at least one iipase in the manufacture of a feedstuff for increasing the availability of at least one nutrient and/or increasing the available metabolic energy from a feed material. 34. The use according to claim 32, wherein the at ieast one phytase and at least one Iipase are formulated as a feed supplement. 35. The use according to claim 33 wherein the feed supplement is the feed supplement of any one of claims 1 to 15. 36. The use according to any one of claims 32 to 34, wherein the at least one nutrient is selected from phosphorous; calcium; amino acids; fat and/or starch. 37. A feed supplement comprising a phytase and a Iipase substantially as herein described. 38. A feedstuff comprising a feed supplement or at least one phytase and at least one lipase substantially as described herein. 39. A method of making a feedstuff substantially as described herein. 40. A use of at least one phytase and at least one lipase or of a feed supplement to produce a feedstuff substantially as described herein

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