Compositions and methods relating to lipase/acyltransferase enzymes identified in prokaryotes and eukaryotes are described. These enzymes can be used in such applications as lipid stain removal from fabrics and hard surfaces and chemical synthesis reactions.
CLAIMS What is claimed is: 1. A recombinant lipase/acyltransferase enzyme having only limited amino acid sequence identity to Candida albicans CaI-L lipase/acyltransferase, comprising: a) a first amino acid sequence motif GX<1>SX<2>G at residues corresponding to positions 192-196 of the Cpa-L amino acid sequence (SEQ ID NO: 8), where X<1> is an aromatic amino acid and X<2> is an amino acid selected from the group consisting of G, E, or Q; b) a second amino acid sequence motif YAX<1>X<2>Xs, at residues corresponding to positions 210-214 of the Cpa-L amino acid sequence (SEQ ID NO: 8), where X<1> is P or K, X<2> is an acidic amino acid, and X<3> is a non-polar aliphatic amino acid; c) lipase/esterase activity based on hydrolysis of /?-nitrophenylbutyrate in an aqueous solution. 2. The lipase/acyltransferase enzyme of claim 1, having less than about 50% amino acid sequence identity to CaI-L lipase/acyltransferase having the amino acid sequence of SEQ ID NO: 8. 3. The lipase/acyltransferase enzyme of claim 1, having a precursor amino acid sequence of at least 390 amino acid residues. 4. The lipase/acyltransferase enzyme of claim 1 , wherein X<1> in the first amino acid sequence motif is selected from the group consisting of Y and H. 5. The lipase/acyltransferase enzyme of claim 1, wherein X<2> in the first amino acid sequence motif is selected from the group consisting of G and Q. 6. The lipase/acyltransferase enzyme of claim 1, wherein the first amino acid sequence motif has a sequence selected from the group consisting of GYSGG, GYSQG, and GHSQG. 7. The lipase/acyltransferase enzyme of claim 1, wherein X<1> in the second amino acid sequence motif is selected from the group consisting of D and E. 8. The lipase/acyltransferase enzyme of claim 1, wherein X<2> in the second amino acid sequence motif is selected from the group consisting of L, V, and I. 9. The lipase/acyltransferase enzyme of claim 1, wherein the second amino acid sequence motif has a sequence selected from the group consisting of YAPEL, YAPDV, YAPDL, YAPEI, and YAKEL. 10. The lipase/acyltransferase enzyme of claim 1, having an amino acid sequence having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 11, SEQ ID NO: 14, SEQ ID NO: 17, SEQ ID NO: 20, SEQ ID NO: 23, SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 32, SEQ ID NO: 35, SEQ ID NO: 38, SEQ ID NO: 41, SEQ ID NO: 44, SEQ ID NO: 47, SEQ ID NO: 50, and SEQ ID NO: 53. 11. The lipase/acyltransferase enzyme of claim 1, with the provisio that the lipase/acyltransferase enzyme does not have the amino acid sequence of SEQ ID NO: 5 or SEQ ID NO: 8. 12. The lipase/acyltransferase enzyme of claim 1, wherein the lipase/acyltransferase enzyme is selected from the group consisting of Aad-L, Pst-L, Sco-L, Mfu-L, Rsp-L, Cje-L, Ate-L, Aor-L-0488, Afu-L, Ani-L, AcI-L, Aor-L-6767, Fve-L, Fgr-L, Ksp-L, and Dha-L. 13. The lipase/acyltransferase enzyme of claim 1, with the provisio that the lipase/acyltransferase enzyme is not CaI-L or CpaL. 14. A recombinant lipase/acyltransferase enzyme having at least 90% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 11, SEQ ID NO: 14, SEQ ID NO: 17, SEQ ID NO: 20, SEQ ID NO: 23, SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 32, SEQ ID NO: 35, SEQ ID NO: 38, SEQ ID NO: 41, SEQ ID NO: 44, SEQ ID NO: 47, SEQ ID NO: 50, and SEQ ID NO: 53. 15. A composition comprising the lipase/acyltransferase enzyme of claim 1 16. The composition of claim 14, wherein the lipase/acyltransferase enzyme is expressed in a heterologous host cell. 17. The composition of claim 15, wherein the composition is a detergent composition, and the lipase/acyltransferase enzyme is Sco-L. 18. A composition comprising a recombinant lipase/acyltransferase enzyme having at least 90% amino acid sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 11, SEQ ID NO: 14, SEQ ID NO: 17, SEQ ID NO: 20, SEQ ID NO: 23, SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 32, SEQ ID NO: 35, SEQ ID NO: 38, SEQ ID NO: 41, SEQ ID NO: 44, SEQ ID NO: 47, SEQ ID NO: 50, and SEQ ID NO: 53. 19. A method for removing an oily soil or stain from a surface, comprising contacting the surface with a composition comprising the lipase/acyltransferase enzyme of claim 1. 20. The method of claim 19, wherein the composition is a detergent composition and the lipase/acyltransferase is Sco-L. 21. The method of claim 19, wherein the surface is a textile surface. 22. A method for forming a peracid comprising contacting an acyl donor and hydrogen peroxide with the lipase/acyltransferase enzyme of claim 1. 23. The method of claim 22, wherein the lipase/acyltransferase enzyme is Aad-L. 24. A method for forming an ester surfactant comprising contacting an acyl donor and acceptor with the lipase/acyltransferase enzyme of claim 1. 25. The method of claim 24, wherein the lipase/acyltransferase enzyme is Aad-L, Pst- L, Sco-L, or Mfu-L. 26. A method for making biodiesel, comprising contacting an acyl donor and acceptor with the lipase/acyltransferase enzyme of claim 1. 27. The method of claim 26, wherein the lipase/acyl transferase enzyme is Aad-L or Pst-L. 28. The method of any one of claims 19-27, wherein the lipase/acyltransferase enzyme is expressed in a heterologous host cell. 29. An expression vector comprising a polynucleotide encoding the lipase/acyltransferase enzyme of claim 1 and a signal sequence to cause secretion of the lipase/acyltransferase enzyme. 30. An expression vector comprising a polynucleotide encoding the lipase/acyltransferase enzyme CaI-L or Cpa-L and a signal sequence to cause secretion of the lipase/acyltransferase enzyme. 31. A method for expressing a lipase/acyltransferase enzyme, comprising: introducing the expression vector of claim 29 into a suitable host, expressing the lipase/acyltransferase enzyme, and recovering the lipase/acyltransferase enzyme expressed. 32. A method for expressing a lipase/acyltransferase enzyme, comprising: introducing the expression vector of claim 30 into a suitable host, expressing the lipase/acyltransferase enzyme, and recovering the lipase/acyltransferase enzyme expressed.