Resumen
The present invention includes with the next stage: the size that the lignin fiber cellulosic biomass is crushed to 15-30mm, the product that is obtained carried out 1-10 minute vapor explosion pre-treatment under 190-230 ℃ in reactor (2), in whirlwind splitter (3), collect pretreated material and in pressure filter (9), pass through filtering separation liquid and solid part, solid part is introduced in the fermentation storage (10), adding concentration is the beta-glucosidase that 15UFP/ restrains cellulosic cellulase and 12.6 international unit, said cellulase and beta-glucosidase are dissolved in the citrate buffer of pH4.8, give the culture of fermentation storage (10) inoculation Marx Crewe Vickers yeast CECT10875 heat resistant type bacterial strain, this bacterial strain obtains by chemomorphosis Marx Crewe Vickers zymic DER-26 bacterial strain, and mixture was shaken under 42 ℃ 72 hours.
Reivindicaciones
1. Procedure for the production of ethanol from lignocellulosic biomass characterised in that it includes the stages of: Grinding the lignocellulosic biomass Subjecting the ground lignocellulosic biomass to steam explosion pre-treatment, maintaining at a pressure of between 1 and 3 MPa and a temperature between 190 and 230°C, for a period of time of between 1 and 10 minutes, depending on the type of material used and later provoking rapid de-pressurisation. Collecting the pre-treated material and separating the liquid and solid fractions by filtration, and introducing the solid fraction in the fermentation deposit (10). Adding a cellulase to the fermentation deposit (10) in a concentration of 15 UFP per gram of cellulose and 12.6 International Units of β-glucosidase enzyme. Inoculating the fermentation deposit (10) with a suspension of a culture of the heat-tolerant yeast Kluyveromyces marxianus CECT 10875. Shaking the mixture for 72 h at 42°C. Determining the concentration of ethanol and residual sugars in the mix, once the reaction is complete. 2. Procedure for the production of ethanol from lignocellulosic biomass, in accordance with claim 1, characterised in that the particle size of the lignocellulosic biomass after grinding is between 15 and 30 mm. 3. Procedure for the production of ethanol from lignocellulosic biomass, in accordance with claim 1, characterised in that the culture of Kluyveromyces marxianus CECT 10875 is obtained by chemical mutagenesis from the DER-26 strain of Kluyveromyces marxianus. 4. Procedure for the production of ethanol from lignocellulosic biomass, in accordance with claim 1, characterised in that the humidity content of the lignocellulosic biomass is between 10 and 15%. 5. Procedure for the production of ethanol from lignocellulosic biomass, in accordance with claim 1, characterised in that the solid fraction that is introduced into the fermentation deposit has a solid/liquid ratio that varies between 8 and 15% (w/v). 6. Procedure for the production of ethanol from lignocellulosic biomass, in accordance with claim 1, characterised in that the cellulase is CELLUCLAST 1.5L, from the NOVO-NORDISK company, and the β-glucosidase enzyme is NOVOZYME 188, from the NOVO-NORDISK company. 7. Procedure for the production of ethanol from lignocellulosic biomass, in accordance with claim 1, characterised in that the cellulase and the β-glucosidase are dissolved in citrate buffer pH 4.8. 8. Procedure for the production of ethanol from lignocellulosic biomass, in accordance with claim 1, characterised in that the Kluyveromyces marxianus CECT 10875 inoculant is at a concentration of 10% v/v. 9. Procedure for the production of ethanol from lignocellulosic biomass, in accordance with claim 1, characterised in that the mixture is shaken at 150 r.p.m.
