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METHOD FOR INCREASING PATHOGEN RESISTANCE IN PLANTSCM Patents

Índice de la ficha

Updated at
24/07/2026
Numero publicacion
WO.2014095990.A1
Fecha publicacion
26/06/2014
Numero solicitud
WO2013EP77076
Fecha presentacion
18/12/2013

En detalle

Resumen

[0001] The invention relates to a method of increasing the resistance to one or more pathogens, preferably fungal or bacterial pathogens, in a monocotyledonous or dicotyledonous plant or a part of a plant, for example in an organ, tissue, a cell or a part of a plant cell, for example in an organelle, wherein a DNA sequence which codes for YODA protein or wherein an endogenous DNA sequence which codes for a YODA protein is increased in the plant or plant cell in comparison with the original, or wild-type, plant. The invention also relates to plants, to parts of a plant, for example an organ, tissue, a cell or a part of a plant cell, for example an organelle, which are obtained by the above methods, and to the corresponding propagation material.

Reivindicaciones

1. A method for increasing fungal resistance in a plant, a plant part, or a plant cell, characterised in that the method comprises the step of increasing the expression and / or activity of a YODA protein in the plant, plant part, or plant cell in comparison to a wild type plant, wild type plant part or wild type plant cell. The method according to claim 1 , characterised in that said YODA protein comprises an amino acid sequence having at least 80% identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 69 or 72. The method according to claims 1 or 2, comprising introducing an exogenous nucleotide sequence which codes for an amino acid sequence having at least 80% identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 69 or 72 into a plant, a part of a plant, or a plant cell, and expressing said nucleotide sequence in the plant, the part of the plant, or the plant cell; wherein said nucleotide sequence is increased in the plant, the part of the plant, or the plant cell in comparison with the original, or wild-type plant, part of the plant, or plant cell. The method according to claims 1 or 2, comprising modifying an endogenous nucleotide sequence which codes for an amino acid sequence having at least 80% identity with SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 69 or 72, and/or the 5'-untranslated region (5'UTR) in comparison with the original sequence. The method according to claim 3, characterised in that said nucleotide sequence comprises at least one nucleic acid molecule selected from the group consisting of: a) an exogenous nucleic acid molecule which codes for at least one polypeptide comprising the sequence as shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 69 or 72; b) an exogenous nucleic acid molecule which comprises at least one polynucleotide of the sequence as shown in SEQ ID NO: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25, 27, 29, 31 , 33, 35, 37, 39, 41 , 43, 45, 47, 70 or 71 ,; c) an exogenous nucleic acid molecule which codes for a polypeptide whose sequence has at least 80% identity to the sequence as shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 69 or 72; d) an exogenous nucleic acid molecule which comprises at least one polynucleotide whose sequence has at least 80% identity to the sequence as shown in SEQ ID NO: 1 , 3, 5, 7, 9, 1 1 , 13, 15, 17, 19, 21 , 23, 25, 27, 29, 31 , 33, 35, 37, 39, 41 , 43, 45, 47, 69, 70 or 71 ; e) an exogenous nucleic acid molecule according to (a) to (d) which codes for a fragment or an epitope of the sequence as shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 69 or 72; f) an exogenous nucleic acid molecule which codes for a polypeptide which is recognized by a monoclonal antibody directed against a polypeptide which is encoded by the nucleic acid molecule as shown in (a) to (d); g) an exogenous nucleic acid molecule which hybridizes under stringent conditions with the complement of the nucleic acid molecule as shown in (a) to (d); and h) an exogenous nucleic acid molecule which can be isolated from a DNA library using the complement of the nucleic acid molecule as shown in (a) to (d) or their part-fragments of at least 15 nt, 20 nt, 30 nt, 50 nt, 100 nt, 200 nt or 500 nt, as probe under stringent hybridization conditions; the nucleotide sequence being such that a pathogen resistance is generated or increased thereby; i) an exogenous nucleic acid molecule of (a) to (d) above, but differing the nucleic acid molecule of (a) to (d) above due to the degeneracy of the genetic code. 6. The method according to any one of claims 1 to 6, characterised in that a fungal or a bacterial pathogen resistance is generated or increased. 7. The method according to claim 6, characterised in that the fungal pathogen is a necrotroph, a biotroph or an Oomycete pathogen. 8. The method according to any one of claims 3 or 5-7, comprising a) introducing into a plant cell a recombinant expression cassette comprising the nucleic acid molecule as characterized in (a) to (i) in an operable linkage with a promoter which is active in plants; b) regenerating a plant from the plant cell, and c) expressing said nucleic acid molecule to generate or to increase a pathogen resistance in said plant. 9. The method according to claim 8, characterised in that the promoter is a pathogen-inducible promoter or an epidermis, or mesophyll-specific promoter, or the promoter is a stress induced promoter; preferably when the promoter is selected from the group consisting of a promoter induced by: osmotic stress, drought stress, cold stress, heat stress, oxidative stress, nutrient deficiency, infection by a fungus, infection by an oomycete, infection by a virus, infection by a bacterium, nematode infestation, pest infestation, weed infestation, and herbivory. 10. The method according to any one of claims 1 -9, characterised in that the plant is selected from the group consisting of: soybean, potato, cotton, rape, oilseed rape, canola, sunflower, alfalfa, clover, banana, blackberry, blueberry, strawberry, raspberry, cantaloupe, carrot, cauliflower, coffee, cucumber, eggplant, grape, honeydew, lettuce, mango, melon, onion, papaya, pepper, pineapple, pumpkin, spinach, squash, tobacco, tomato, tomatillo, watermelon, apple, peach, pear, cherry, plum, broccoli, cabbage, cauliflower, Brussels sprouts, kohlrabi, currant, avocado, orange, lemon, grapefruit, tangerine, artichoke, cherry, walnut, peanut, endive, leek, arrowroot, beet, cassava, turnip, radish, yam, sweet potato; pea, bean, sugarcane, turfgrass, Miscanthus, switchgrass, wheat, maize, sweet com, rice, millet, sorghum, barley, and rye. 1 1 . The method according to claim 5, characterised in that said nucleotide sequence is in operable linkage with a pathogen-inducible promoter or an epidermis- and/or mesophyll- specific promoter. 12. A recombinant DNA expression cassette comprising a nucleic acid molecule as characterized in claim 5. 13. The recombinant DNA expression cassette according to claim 12, characterised in that said nucleic acid sequence is in operable linkage with a promoter which is functional in plants. 14. A recombinant vector comprising the expression cassette of claim 12. 15. A cell comprising the nucleic acid molecule as characterized in claim 5, a DNA expression cassette comprising said nucleic acid molecule, or a vector comprising said expression cassette. 16. A transgenic nonhuman organism or a plant, comprising a nucleic acid molecule as characterized in claim 5, a DNA expression cassette comprising said nucleic acid molecule, or a vector comprising said expression cassette, or comprising a cell comprising said nucleic acid molecule, said expression cassette, or said vector. 17. The organism or the plant according to claim 16, selected from the group consisting of soybean, potato, cotton, rape, oilseed rape, canola, sunflower, alfalfa, clover, banana, blackberry, blueberry, strawberry, raspberry, cantaloupe, carrot, cauliflower, coffee, cucumber, eggplant, grape, honeydew, lettuce, mango, melon, onion, papaya, pepper, pineapple, pumpkin, spinach, squash, tobacco, tomato, tomatillo, watermelon, apple, peach, pear, cherry, plum, broccoli, cabbage, cauliflower, Brussels sprouts, kohlrabi, currant, avocado, orange, lemon, grapefruit, tangerine, artichoke, cherry, walnut, peanut, endive, leek, arrowroot, beet, cassava, turnip, radish, yam, sweet potato; pea, bean, sugarcane, turfgrass, Miscanthus, switchgrass, wheat, maize, sweet com, rice, millet, sorghum, barley, and rye. 18. A method for generating a transgenic plant which is resistant to oomycetes and/or fungi and /or bacterial pathogens, comprising using the nucleic acid molecule as characterized in claim 5, a DNA expression cassette comprising said nucleic acid molecule, a vector comprising said expression cassette, or a cell comprising said nucleic acid molecule, said expression cassette, or said vector. 19. A crop, propagation material or composition comprising the nucleic acid molecule as characterized in claim 5, a DNA expression cassette comprising said nucleic acid molecule, or a vector comprising said expression cassette, or comprising a cell comprising said nucleic acid molecule, said expression cassette, or said vector. 20. Use of any of the nucleic acids molecules described in claims 5, the DNA expression cassette according to claim 13 or 14, or the vector according to claim 15 for the transformation of a plant, plant part, or plant cell to provide a fungal resistant plant, plant part, or plant cell. 21 . Harvestable part of a transgenic nonhuman organism or plant described in claim 16 or 17, wherein the harvestable part is preferably a transgenic seed of the transgenic plant. 22. Product derived from a nonhuman organism according to claim 16 or plant according to claim 18, from a plant producible by the method of any one of claims 1 -10 or 18 or from the harvestable part of the plant according to claim 21 , wherein the product is preferably soybean meal or soy oil. 23. Method for the production of a product comprising a) growing a plant according to claim 16 or 17 or obtainable by the method according to any one of claims 1 -10 or 18 and b) producing said product from or by the plant and/or part, preferably seeds, of the plant. 24. Method according to claim 23 comprising a) growing a plant according to claim 16 or 17 or obtainable by the method according to any one of claims 1 -10 or 18 and removing the harvestable parts as defined in claim 21 from the plants; and b) producing said product from or by the harvestable parts of the plant. 25. Method according to claims 23 or 24, wherein the product is meal or oil, preferably, soybean meal or soybean oil.

Etiquetas

Inventores
Lucia Jorda MiroClara Sanchez RodriguezSara Sopea TorresEva Miedes VicenteAndres Sanchez ValletViviana Pamela Escudero WelschAntonio Molina FernandezGemma Lopez GarciaMolina Fernandez AntonioJorda Miro LuciaSanchez Rodriguez ClaraSopena Torres SaraLopez Garcia GemmaMiedes Vicente EvaSanchez Vallet AndreaEscudero Welsch Viviana PamelaMolina Fernández AntonioJordá Miro LucíaSánchez Rodríguez ClaraLópez García GemmaSánchez Vallet Andrea
Solicitantes
Universidad Politécnica de Madrid
Clasificacion ipc
A01H 1/ 00 A IA01H 5/ 00 A IC12N 15/ 82 A IC12N 5/ 14 A IC12N 9/ 12 A I
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