Resumen
Optical detection system for labelling-free high-sensitivity bioassays, which comprises the use of optical characterization techniques that allow vertical interrogation in micrometric and sub-micrometric domains and also at least one vertical-interrogation biophotonic interferometric or resonant cell, characterized in that said system further comprises at least: (i) an optical measuring system that comprises, in turn, and at least, one excitation source, optical means for detection of the signal originating from an optical head, and an optical head; and (ii) an element for the integration of multiple analytes, comprising, in turn, and at least, a plurality of biosensitive cells, a plurality of fluid connections connected with the cells, and a substrate on which rest not only the biosensitive cells but also the fluid connections. In said system, said optical head is arranged in such a manner as to allow analysis in respect of each one of said biosensitive cells and the analytes contained therein.
Reivindicaciones
1. Optical detection system for high sensitivity labelling-free bioassays comprising the use of optical characterization techniques that allow the vertical interrogation of micro and sub-micrometric domains as well as at least one resonant or interferometric biophotonic cell of vertical interrogation characterized in that it also comprises, at least: (i) an optical measuring system that comprises, in turn, and at least an excitation source, optical means for the detection of a signal coming from an optical head; and an optical head; and (ii) an element for the integration of multiple analytes comprising, in turn and at least, a plurality of biosensitive cells, a plurality of fluid connections connected to the cells; and a substrate on which both the biosensitive cells and the fluid connections rest; and where said optical head is arranged to analyze each one of said biosensitive cells and the analytes contained therein. 2. A system according to claim 1 characterized in that said excitation sources are laser-type sources or other sources coherent or not in visible and infrared wavelengths. 3. A system according to claims 1 and 2 characterized in that the element of integration of multiple analytes is a large substrate, of the silicon-, transparent glass- or the like wafer-type. 4. A system according to claims 1 and 2 characterized in that the element of integration of multiple analytes is a disposable biochip. 5. A system according to claims 1, 2, 3 or 4 characterized in that the biosensitive cell of vertical interrogation is based on interferometric and resonant phenomena sensitive to molecular recognition when they are vertically interrogated. 6. A system according to the preceding claims characterized in that the biosensitive cell is based on a resonant nano-cavity with interrogation by reflection or transmission, with reflectivity on the basis of the incidence angle, wavelength and where the phase difference is simultaneously obtained and where said biosensitive cell comprises, at least: (a) a plurality of Bragg reflectors; (b) a resonant cavity; (c) a plurality of biomolecules, which enter through a fluid inlet and exit through a fluid outlet; being, besides, the plurality of Bragg reflectors, the resonant cavity and the plurality of biomolecules, deposited on a substrate. 7. A system according to claims 1, 2 and 3 or 4 characterized in that the biosensitive cell is based on resonant micropillars of interrogation by reflection or transmission and comprises, at least: (a) a plurality of resonant cavities; (b) a plurality of antibodies/antigen attached to the surface of the resonant cavities; (c) a plurality of Bragg reflectors, at least two per resonant cavity; being said elements also deposited on a substrate. 8. A system according to claims 1, 2, 3 or 4, 5 and 6 or 7 characterized in that in interrogation by transmission, the optical head is an optical transmitter head connected to the excitation source whose beam is received by an optical receptor head, connected to the optical detection means. 9. A system according to claims 1, 2, 3 or 4, 5 and 6 or 7 characterized in that in interrogation by reflection, the optical head is a transmitter-receptor.
