Resumen
The present invention refers to an isolated nucleic acid molecule for expression of a gene in seeds, having promoter activity, comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1, a first sequence at least 70% homologous to SEQ ID NO:1, with the functionality of SEQ ID NO:1, a second sequence at least 70% homologous to the sequence complementary to SEQ ID NO:1, with the functionality of SEQ ID NO:1, and fragments thereof, with the functionality of SEQ ID NO:1; to chimeric genes, constructs, vectors, expression cassettes, host cells and transgenic plants comprising said sequences; as well as to a method for expression of a gene specifically in seeds or seed parts using one of the mentioned sequences or chimeric genes comprising said sequence s and to a method for obtaining substances through the transferring of the mentioned chimeric genes to a plant and expressing said chimeric gene.
Reivindicaciones
1. Nucleotide sequence of the Ha ds10 G1 sunflower gene, including its promoter and specific regulatory elements of seeds, described by SEQ N° 1, and by the restriction maps in Figure 1; and characterized in Examples 1-3. 2. The sequences, or part of them, identical or homologous to SEQ N°1 or its complementary sequence (at least by 70%, for example by 80% and particularly less than 95%) 3. Genes that contain the sequences mentioned in claims 1-2 and that are specifically expressed in seeds, in a homogeneous and abundant manner, from early stages of maturation. These genes may be constructed and used by recombinant DNA techniques, according to the details in the following claims (3-6): 4. Use in order to confer specific expression in seeds, by means of recombinant DNA techniques, of the Ha ds10 G promoter and 5'-flanking and coding sequences 1 (or part of said sequences), contained in the constructions: ds10F1, ds10F2 ds10F2Δ, ds10F3 and ds10EC1 (described in Figure 5). 5. The use of Ha ds10 G1 coding and 5'-flanking sequences (or part of said sequences), contained in the constructions ds10F2 and ds10FΔ, in order to increase chimeric gene expression specifically in transgenic plant seeds. 6. The use of coding and intron sequences of Ha ds10 G1 (or part of said sequences), contained in the construction ds10F3, in order to increase the expression of other chimeric genes in seeds, and/or to reduce it in other tissues, thus increasing the effectiveness and specificity in seeds of these chimeric genes. 7. Add to the above: seed, part of the seed and seed extract. 8. Expression cassette that contains a sequence described in claims 1 to 6. 9. Vector(s) that contains(contain) a sequence described in claims 1 to 7. 10. Host cells that contain a sequence described in claims 1 to 7. 11. The process of obtention of transgenic plants characterized in the transformation of a plant (for example, sunflower, soybean, oilseed rape, "canola", maize, wheat, barley, rice, bean, cassava, peanut, tobacco, etc.), with an expression cassette described in claim 8. 12. Production procedures, for example of coil, proteins or of bioactive substances, by using transgenic plants such as the ones described in claim 11. 13. Products, for example, oil, proteins or bioactive substances, obtained according to claim 12.
