The invention concerns an enzymatic process for detaching or facilitating removal of biofilm that has accumulated during solid-liquid-air interfaces and solid-liquid interfaces of aqueous systems. This is achieved by exposing the biofilm to a cleaning preparation containing at least one enzyme pertaining to the carbohydrase and protease group. Sequential treatment of the biofilm initially using a cleaning preparation essentially containing carbohydrase and then using a cleaning preparation essentially containing protease makes it possible to eliminate up to 85 % of accumulated biomass and up to 100 % of adhered cells.