madrid2i

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[0001] The invention relates to a method for producing 2,3-butanediol using improved strains of Raoultella planticola, and to novel mutant strains obtained by random mutagenesis from the bacterial species Raoultella planticola CECT843, that can be used in the industrial production of 2,3-butanediol from glycerol. The invention preferably relates to the Raoultella planticola strains designated IA1 and IIIA3 and deposited in the Spanish Type Culture Collection (CECT) under deposit number CECT8158 (corresponding to the strain designated IA1) and deposit number CECT8159 (corresponding to the strain designated IIIA3). The invention also relates to a method for producing 2,3-butanediol from glycerol by means of a biotechnological process using the novel strains of the invention.

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1. Mutant strains of the speciesRaoultella planticola that can be used in the production of 2,3-butanediol from glycerol obtained by randomly induced mutagenesis of theRaoultella planticola strain CECT843 and subsequent selection in bromate/bromide plates of mutants with an improved 2,3-butanediol production capacity. 2. The mutant strains according to claim 1, selected from the group consisting of the strains ofR .planticola, under accession number CECT8158 and accession number CECT8159, deposited in the Spanish Type Culture Collection (CECT) on 12/06/2012. 3. An industrially viable method for the biotechnological conversion of glycerol into 2,3-butanediol comprising the following steps: a) aerobically fermenting the strains according to the preceding claims in a medium comprising an aqueous solution of glycerol under conditions suitable for producing 2,3-butanediol; and b) separating the 2,3-butanediol produced from the reaction medium. 4. The method according to claim 3, characterized in that the aqueous solution of glycerol contains at least 3% by weight of glycerol. 5. The method according to claim 4, characterized in that the aqueous solution of glycerol contains at least 6% by weight of glycerol. 6. The method according to claims 3 to 5, characterized in that the reaction temperature of fermentation step a) is 28°C - 37°C. 7. The method according to claims 3 to 6, characterized in that the reaction time of fermentation step a) is 12 to 48 hours. 8. The method according to claims 3 to 7, characterized in that the reaction pH of fermentation step a) is 7.5 to 5.5. 9. The method according to claims 3 to 8, characterized in that the separation of 2,3-butanediol in step b) is done by filtration or centrifugation and subsequent extraction. 10. A method for producing mutant strains of the speciesR .planticola according to the strains claimed in claim 1, comprising randomly induced mutagenesis of theR .planticola strain CECT843 and subsequent selection in bromate/bromide plates of mutants with an improved 2,3-butanediol production capacity. 11. The method according to claim 10, characterized in that the step of randomly induced mutagenesis is performed by means of a chemical mutagen or UV radiation. 12. The method according to claim 11, characterized in that the step of random mutagenesis with chemical mutagen is performed by incubating theR .planticola strain CECT 843 in a culture medium with 30 g/L of glycerol and 10 µL/mL of ethyl methanesulfonate (EMS) for 1 hour at 30°C. 13. The method according to claims 10 to 12, characterized in that the subsequent selection of mutant strains is done by seeding and incubating the colonies resulting from the mutagenesis step in selection plates with 100 mM NaBr and 100 mM NaBrO<3> for 2 to 3 days at pH 6. 14. The method according to claim 13, characterized in that colonies reducing the pH of the medium below 6.4 are discarded in the selection of mutant strains.

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