Resumen
Tool for capturing integron cassettes. The invention relates to a tool for capturing integron cassettes that includes the gene for a counterselective marker into which an integration site (attl) has been inserted to convert it into a blind reading marker for selecting recombination events. The tool also includes the integrase intl gene. The construct of the counterselective marker with the attl site can be integrated into the chromosome of a bacterium, especially a strain of Vibrio cholera lacking a superintegron, while the intl gene is included in a plasmid into which, in addition, the tfoX gene, regulator of the natural competence of V. cholerae, can be inserted. The invention also relates to methods and kits for detecting integron cassettes present in other bacteria, by conjugation, and integron cassettes from DNA samples, by natural transformation. (Machine-translation by Google Translate, not legally binding)