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A COMPOSITION COMPRISING A COUPLED ENZYME SYSTEMCM Patents

The present invention relates to a composition comprising a coupled enzyme system for the rapid and efficient production of hydrogen peroxide by the coupling of a first enzyme system capable of hydrogen peroxide generation, to a second enzyme system which utilizes the non hydrogen peroxide product of the first enzyme system, and optionally is capable of generating further hydrogen peroxide.

Datos clave

CategoríaInvención

Índice de la ficha

Updated at
05/11/2026
Numero publicacion
WO.2007045251.A2
Fecha publicacion
26/04/2007
Anio publicacion
2007
Numero solicitud
2006000590

En detalle

Reivindicaciones

CLAIMS 1. A composition comprising a first oxidase, a first substrate, and at least one further enzyme, wherein, the first substrate is oxidisable by the first oxidase to form hydrogen peroxide and a second substrate; and the second substrate is convertable by the at least one further enzyme to form a product. 2. The composition according to claim 1, wherein the first substrate is a polyol. 3. The composition according to claim 2 or 3, wherein the polyol is a pentitol or a hexitol. 4. The composition according to claim 2 or 3, wherein the polyol is selected from the group consisiting of hexitol, pentitol, sorbitol, xylitol, maltitol, mannitol, galactitol, isomalt, lactitol, arabitol, erythritol, and ribitol. 5. The composition according to claim 4, wherein the polyoi is xylitol. 6. The composition according to claim 4, wherein the polyol is sorbitol, 7. The composition according to any one of claims 1 to 6 wherein the second substrate is a sugar. 8. The composition according to claim 7, wherein the sugar is a monosaccharide or a disaccharide. 9. The composition according to claim 8, wherein the sugar is selected form the group consisting of glucose, xylose, maltose, mannose, galactose, isomaltose, lactose, arabinose, erythrose and ribose. 10. The composition according to claim 9, wherein the sugar is xylose or glucose. 11. The composition according to any one of claims 1 to 10, wherein the first oxidase is a polyol oxidase. 12. The composition according to claim 11, wherein the first oxidase is selected from the group consisting of hexitol oxidase, pentitol oxidase, sorbitol oxidase, xylitol oxidase, maltitol oxidase, mannitol oxidase, galactitol oxidase, isomalt oxidase, lactitol oxidase, arabitol oxidase, arabitol oxidase, erythritol oxidase, and ribitol oxidase. 13. The composition according to claims 11 or 12, wherein the first oxidase has a polyol oxidase (specific) activity of at least 5 units/g protein when using the respective polyol substrate. 14. The composition according to any one of claims 11 to 13, wherein the polyol oxidase is sorbitol oxidase or xylitol oxidase. 15. The composition according to any one of claims 11 to 14, wherein the polyol oxidase has a ratio of specific activity on the respective polyol to the corresponding sugar of greater than 1. 16. The composition according to any one of claims 11 to 15, wherein the polyol oxidase has a ratio of specific activity on the respective polyol to glucose of greater than 1. 17. The composition according to any one of claims 11 to 16, wherein the polyol oxidase has a higher specific activity on sorbitol as compared to xylitol. 18. The composition according to any one of claims 11 to 17, wherein the polyol oxidase is derived from a strain of Streptomyces or Xanthomonas. 19. The composition according to any one claims 1 to 18, wherein the first oxidase comprises a polypeptide sequence SEQ ID NO 1 or SEQ ID NO 2 or a homologue, variant or fragment thereof. 20. The composition according to claim 19, wherein the first oxidase consists of a polypeptide sequence SEQ ID NO 1 or SEQ ID NO 19. 21. The composition according to any one of claims 11 to 20, wherein the level of first oxidase activity present in the composition (or added to the composition) is between about 0.1 and about 200,000 units per kg of said composition. 22. The composition according to any one of claims 1 to 21, wherein the at least one further enzyme is a further oxidoreductase. 23. The composition according to claim 22, wherein the second substrate is oxidisable by the further oxidoreductase to form hydrogen peroxide and said product. 24. The composition according to claim 22 or 23, wherein the further oxidoreductase is a sugar oxidase. 25. The composition according to claim 23 or 24, wherein the sugar-oxidase is selected form the group consisting of: carbohydrate oxidase, oligosaccharide oxidase, maltose oxidase, hexose oxidase, glucose oxidase, mannose oxidase, galactose oxidase, isolmaltulose oxidase, lactose oxidse, arabinose oxidase, erythrose oxidase, pentose oxidase, xylose oxidase, and those oxidase. 26. The composition according to any one of claims 23 - 25 wherein the sugar-oxidase is selected form the group consisting of: EC 1.1.3.4 glucose oxidase, EC 1.1.3.5 hexose oxidase, EC 1.1.3.9 galactose oxidase, EC 1.1.3.10 pyranose oxidase, EC 1.1.3.11 L- sorbose oxidase, and EC 1.1.3.40 D-mannitol oxidase. 27. The composition according to any one claims 22 to 26, wherein the amount of the at least one further oxidoreductase compared to the amount of the first oxidase such as the polyol oxidase present in said composition is greater than 1, as measured by the respective number of enzyme units present in said composition. 28. The composition according to any one of claims 1 to 27, wherein the presence of the product produced from the second substrate does not reduce trie rate of hydrogen peroxide production from the oxidation of the first substrate by the first oxidase. 29. The composition according to any one of claims 1 to 28, wherein the product produced from the second substrate is a lactone, a dialdose or a dehydro sugar. 30. The composition according to any one of claims 1 to 29, wherein the composition is edible. 31. An edible composition comprising the composition according to claim 30. 32. A composition according to any of claims 1 to 31 for use as a medicament. 33. An oral care product comprising a composition according to any one of claims 1-32 and at least one further ingredient used in oral care products 34. An oral care product according to claim 33, wherein the oral care product is in the form selected from the group consisting of: chewing gum, a mouthwash, a mouthspray, a pastille, a lozenge, a mouth freshener, a nasal spray, and an oral paste. 35. An oral care product according to claims 33 or 34, which comprises xylitol and/or sorbitol. 36. A cosmetic product comprising the composition according to any one of claims 1-30 and at least one further ingredient used in cosmetic products 37. A skin, hair or teeth bleaching product comprising the composition according to any one of claims 1-30 and at least one further ingredient used skin, hair or teeth bleaching and/ or whitening products. 38. A cosmetic method for bleaching and/or whitening of external mammalian tissue, comprising contacting the external mammalian tissue with composition according to any one of claims 1-30, or a product according to any one of claims 33 to 37 in an amount and duration suitable for bleaching and/or whitening of the external mammalian tissue. 39. A cosmetic method according to claim 38, wherein the external mammalian tissue is selected from the group consisting of teeth, hair and skin. 40. A detergent or bleaching product, suitable for use on non-living tissue, comprising the composition according to any one of the claims 1-29, and at least one further ingredient used in detergent or bleaching products. 41. A paint product, such as a maritime, decorative or protective paint, which comprises the composition according to any one of claims 1 - 29, and at least one further ingredient used in paint. 42. A pesticide which comprises the composition according to any one of claims 1-29, and at least one further ingredient used in pesticides. 43. The use of a composition according any one of claims 1-30 or an oral care product according to any one of claims 33-35 for the production of hydrogen peroxide. 44. The use according to claim 43, wherein said use occurs in an oral care product to provide beneficial teeth bleaching and/or whitening effects, and/or extended shelf life, and/or anti-microbial/anti-bacterial effects either prior to or during use. 45. The use according to claim 43, wherein said use occurs in an edible product to provide beneficial prebiotic effects when consumed by an individual mammal, and/or a prolonged shelf life. 46. The use according to claim 43, wherein said use occurs in a cosmetic product to provide a prolonged shelf life, and/or is capable of bleaching and/or whitening external mammalian tissue, and/or has an anti-microbial/anti-bacterial effect when applied to the human skin. 47. The use according to claim 43, wherein said use occurs in a detergent to enhance the bleaching, whitening or disinfecting capabilities of the detergent when used on a nonliving material 48. The use according to claim 43, wherein said use occurs in a paint product which shows improved preservation either before or after application. 49. The use according to claim 43, wherein said use occurs in a pesticide product which shows improved ability to prevent, reduce or kill microbial pests. 50. A method for the preparation of a composition comprising admixing a first oxidase as defined in any one of the preceding claims, and a first substrate according to any one of the preceding claims, and at least one further oxidored[upsilon]ctase according to any one of the preceding claims, and a suitable matrix. 51. A method according to claim 50, wherein the matrix is edible. 52. A method according to claim 51, wherein the composition is selected from the group consisting of a medicament, an oral care product, and a beverage. 53. A method according to claim 52, wherein the matrix is a detergent or bleaching ingredient or product. 54. A method according to claim 53, wherein the matrix is a paint, such as a maritime or decorative paint. 55. A method according to claim 54, wherein the matrix is a pesticide matrix 56. The use of a composition as defined in claims 1-30, in the manufacture of a medicament for the treatment or prevention of a medical disorder selected from: gum disease, gingivitis, irritable bowel syndrome, lactose intolerance, colon cancer, high blood cholesterol, high blood pressure, hypertension, infection, inflammation and nutritional deficiencies. 57. A method of medical treatment comprising administering the composition according to any one of claims 1-30, or oral care products according to any one of claims 33 to 35 to a patient in need of treatment or prophylaxis. 58. The method according to claim 57, wherein the method is for the treatment or prevention of a medical disorder selected from: gum disease, gingivitis, irritable bowel syndrome, lactose intolerance, colon cancer, high blood cholesterol, high blood pressure, hypertension, infection, inflammation and nutritional deficiencies. 59. A packaged product comprising the composition according to any one of claims 1 to 30, wherein said composition is maintained in a oxygen limited environment within said packaged product so as to prevent or reduce the generation of hydrogen peroxide within said packaged product. 60. A kit of parts comprising the following components: first enzyme, at least one further enzyme and a first substrate, wherein the first enzyme and the first substrate are isolated from one another. 61. A method of generating hydrogen peroxide, the method comprising admixing a first oxidase, and a first substrate, and at least one further enzyme and a suitable matrix, under conditions suitable for the generation of both hydrogen peroxide and a second substrate from the oxidation of the first substrate due to the activity of the first oxidase, wherein the second substrate is convertable by the at least one further enzyme into a product. 62. The method according to claim 61, wherein the at least one further enzyme is a further oxi do reductase, which converts the second substrate into further hydrogen peroxide and the product. 63. The method according to claims 61 or 62, wherein the admixing step comprises mixing the components of the kit of parts according to claim 60. 64. The method according to claims 61 or 62, wherein said method comprises exposing the composition contained within the packaged product according to claim 59 to an exogenous supply of oxygen. 65. A paint composition comprising a poiyo! oxidase and a first substrate, wherein the first substrate is oxidisable by the polyol oxidase to form hydrogen peroxide. 66. A cosmetic composition comprising a polyol oxidase and a first substrate, wherein the first substrate is oxidisable by the polyol oxidase to form hydrogen peroxide. 67. A food or feed composition comprising a polyol oxidase and a first substrate, wherein the first substrate is oxidisable by the polyol oxidase to form hydrogen peroxide. 68. The food or feed composition according to claim 59, wherein said food or feed composition is selected from the group consisting of: Dairy products, such as milk, cream, cheese, whey; beverages, such as fruit juice, 69. A medicament composition comprising a polyol oxidase and a first substrate, wherein the first substrate is oxidisable by the polyol oxidase to form hydrogen peroxide. 70. A medicament composition according to claim 66, wherein the first substrate is xylitol. 71. A pesticide composition comprising a polyol oxidase and a first substrate, wherein the first substrate is oxidisable by the polyol oxidase to form hydrogen peroxide.

Etiquetas

Inventores
Rand, ThomasMadrid, Susan, MampustiRand ThomasMadrid Susan Mampusti
Solicitantes
Danisco US Inc.Dupont Nutrition Biosciences ApSDanisco
Clasificacion ipc
A01N 63/ 50 A IA23L 3/ 3571 A IA61K 38/ 44 A IA61K 8/ 66 A IA61Q 11/ 00 A IC09D 5/ 14 A IC09D 5/ 16 A IA61P 1/ 02 A IA61Q 11/ 02 A I
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