Resumen
The present invention relates to a new strain of mycobacterium avium subspecies paratuberculosis that has undergone a deletion of 16.3 kb for which it lacks genes mce (mammalian cell entry). It also refers to a method developed based on the polymerase chain reaction to detect other strains of mycobacterium avium subspecies paratuberculosis that lack such genes. In addition, the validation of methodologies for the use of this strain of mycobacterium avium subspecies paratuberculosis cect 7530 in in vitro invasion assays in different cell lines of diverse origins is reflected in the present invention. Additionally, the present invention refers to the development of methods for the measurement of the viability of mycobacterium avium subspecies paratuberculosis inside eukaryotic cells during the invasion processes in vitro by estimating the number of copies of the 16s rrna precursor gene. -arnr 16s. (Machine-translation by Google Translate, not legally binding)