Reivindicaciones
1. Claims What is claimed is: 1. An intracellular delivery system comprising: a laser-activated surface; cells positioned at a distance from the laser-activated surface; and a laser providing a laser pulse that enables the cells to import and/or export cargo from or to a medium surrounding the cells. 2. The intracellular delivery system of claim 1, wherein the cells flow into and out of a space adjacent to the laser-activated surface. 3. The intracellular delivery system of claim 1, wherein the cells flow in a continuous flow. 4. The intracellular delivery system of claim 1, wherein the cells are stationary. 5. The intracellular delivery system of claim 1, wherein cargo delivered is initially confined to a layer within liquid adjacent to the laser- activated surface and adjacent to a cell layer. 6. The intracellular delivery system of claim 1, wherein the laser-activated surface is selectively targeted to porate specific cells. 7. The intracellular delivery system of claim 6, wherein the laser- activated surface is spatially targeted. 8. The intracellular delivery system of claim 6, wherein the laser- activated surface is temporally targeted. 9. The intracellular delivery system of claim 1, wherein transport of cargo into or out of the cells subsequent to poration is further promoted by one or more of additional laser pulses, electric fields, turbulent flow, or thermal pulses. 10. The intracellular delivery system of claim 1, further comprising contrast-agent type microbubbles. 11. The intracellular delivery system of claim 10, wherein the contrast-agent type microbubbles are added to the liquid surrounding the cells. 12. The intracellular delivery system of claim 10, wherein a first set of microbubbles is laser- generated and then imploded by a second set of laser-generated bubbles. 13. The intracellular delivery system of claim 1, wherein the laser pulse is provided by an optical source comprising diode-pumped solid state lasers, lamp-pumped solid state lasers, gas lasers, fiber lasers, diode lasers, or quantum cascade lasers. 14. The intracellular delivery system of claim 13, wherein the laser pulse is provided by a continuous wave laser. 15. The intracellular delivery system of claim 13, wherein the laser pulse is produced from a Q-switched, directly modulated, or chopped/redirected light source. 16. The intracellular delivery system of claim 1, wherein cargo is selected from the group consisting of Cas9, dCas, Cas9 RNP, dCas RNP, episomal vectors, TALEN, ZFN, guide RNA, ssODN, mRNA, pre-mRNA, BACs, PNA, pDNA, chromosomes, mitochondria, siRNA, shRNA, miRNA, proteins, morpholinos, metabolites, small molecules, peptides, anitbodies, nanobodies, carbon nanotubes, fluorescent tags and/or dyes, molecular beacons, DNA origami, nanodevices, MEMS devices, polymer constructs including controlled compound release structures, metal or other functional nanoparticles, nuclei, subcellular organelles, ribozymes, enzymes, microbial pathogens, microbeads, surface Raman-enhanced particles, quantum dots, radionuclide, and magnetic beads. 17. The intracellular delivery system of claim 1, wherein the laser-activated surface is patterned. 18. The intracellular delivery system of claim 17, wherein the laser- activated surface comprises a patterned layer of material selected from the group consisting of a thin layer of metal, polymer materials, ink-containing polymers, and an oxide layer. 19. A method of intracellular delivery comprising: positioning a laser-activated surface at a distance from cells; and applying a laser pulse to the surface to porate membranes of the cells to deliver or extract cargo from the cells into a liquid surrounding the cells. 20. The method of claim 19, wherein the cells flow into and out of a space adjacent to the laser-activated surface. 21 The method of claim 19, wherein the cells flow in a continuous flow. 22. The method of claim 19, wherein the cells are stationary. 23. The method of claim 19, wherein cargo delivered is initially confined to a layer within liquid adjacent to the laser-activated surface and adjacent to a cell layer. 24. The method of claim 19, further comprising selectively targeting the laser-activated surface to porate specific cells. 25. The method of claim 24, wherein the laser- activated surface is spatially targeted. 26. The method of claim 24, wherein the laser-activated surface is temporally targeted. 27. The method of claim 19, wherein transport of cargo into or out of the cells subsequent to poration is promoted by applying one or more of additional laser pulses, electric fields, turbulent flow, or thermal pulses. 28. The method of claim 19, further comprising adding contrast-agent type microbubbles to the liquid surrounding the cells. 29. The method of claim 28, further comprising laser-generating a first set of microbubbles and then imploding the first set of microbubbles by a second set of laser- generated bubbles. 30. The method of claim 19, wherein the laser pulse is provided by an optical source comprising diode-pumped solid state lasers, lamp-pumped solid state lasers, gas lasers, fiber lasers, diode lasers, or quantum cascade lasers. 31. The method of claim 30, wherein the laser pulse is provided by a continuous wave laser. 32. The method of claim 30, wherein the laser pulse is produced from a Q-switched, directly modulated, or chopped/redirected light source. 33. The method of claim 19, wherein cargo is selected from the group consisting of Cas9, dCas, Cas9 RNP, dCas RNP, TALEN, ZFN, guide RNA, episomal vectors, ssODN, mRNA, pre- mRNA, BACs, PNA, pDNA, chromosomes, mitochondria, siRNA, shRNA, miRNA, proteins, morpholinos, metabolites, small molecules, peptides, anitbodies, nanobodies, carbon nanotubes, fluorescent tags and/or dyes, molecular beacons, DNA origami, nanodevices, MEMS devices, polymer constructs including controlled compound release structures, metal or other functional nanoparticles, nuclei, subcellular organelles, ribozymes, enzymes, microbial pathogens, microbeads, surface Raman-enhanced particles, quantum dots, radionuclide, and magnetic beads.
