Resumen
IDENTIFICATION AND ISOLATION OF MULTIPOTENT CELLS FROM NON OSTEOCHONDRAL MESENCHYMAL TISSUE Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue. This invention relates to the identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue. Specifically, it relates to an adult multipotent cell or a cell population or composition comprising said cell, isolated from non-osteochondral mesenchymal tissue, characterized in that it is positive for the following markers: CD9, CD10, CD13, CD29, CD44, CD49A, CD51, CD54, CD55, CD58, CD59, CD90 and CD105 and because it lacks expression of the following markers: CD11b, CD14, CD15, CD16, CD31, CD34, CD45, CD49f, CD 102, CD 104, CD 106 and CD133.
Reivindicaciones
1. An isolated multipotent adult cell, which (a) is isolated from non-osteochondral mesenchymal tissue, (b) expresses CD9<+>, CD10<+>, CD13<+>, CD29<+>, CD44<+>, CD49A<+>, CD51 <+>, CD54<+>, CD55<+>, CD58<+>, CD59<+>, CD90<+> and CD105<+>; and (c) lacks expression of CD11b, CD14, CD15, CD16, CD31, CD34, CD45, CD49f, CD102, CD104, CD106 and CD133. 2. An isolated multipotent adult cell according to claim 1, which is isolated by a method that comprises: (a) collecting a non-osteochondral mesenchymal tissue; (b) obtaining a cell suspension by enzymatic digestion; (c) sedimenting and resuspending the cells in a culture medium; and (d) culturing the cells on a solid surface, and eliminating cells that show no adhesion to said solid surface. 3. The isolated multipotent adult cell according to claim 1 or 2, wherein the non-osteochondral mesenchymal tissue is connective tissue. 4. The isolated multipotent adult cell according to claim 3, wherein said connective tissue is adipose tissue. 5. A cell that expresses at least one characteristic of a specialized cell, wherein the cell is derived from an isolated multipotent adult cell according to any of claims 1 to 4. 6. The cell according to claim 5, wherein the at least one characteristic is that of a cell selected from the group consisting of an epithelial cell, an endothelial cell, an adipocyte, a myocyte, a chondrocyte, an osteocyte, a neuron, an astrocyte, an oligodendrocyte, a hepatocyte, a cardiomyocyte, and a pancreatic cell. 7. An isolated cell population that comprises cells according to any of claims 1 to 6. 8. A method for isolating a population of multipotent adult cells according to claim 1, which comprises: (a) collecting a non-osteochondral mesenchymal tissue; (b) obtaining a cell suspension from the tissue by enzymatic digestion; (c) incubating the cell suspension with a labelled compound that binds specifically to one or more of the surface makers of said multipotent adult cells; and (d) selecting those cells that have the desired profile of expression of markers. 9. The method according to claim 8, wherein a negative selection is performed, whereby cells are excluded that show binding to labelled compounds that bind specifically to a marker selected from the group consisting of CD11b, CD14, CD15, CD16, CD31, CD34, CD45, CD49f, CD102, CD104, CD106 and CD133. 10. A substantially homogeneous cell composition which comprises a multipotent adult cell according to any of claims 1 to 4 or a cell population according to claim 7. 11. A pharmaceutical composition that comprises a multipotent adult cell according to any of claims 1 to 4, or a cell population according to claim 7, or obtained according to the method of any of claims 8 or 9, and a pharmaceutically acceptable carrier. 12. An isolated multipotent adult cell according to any of claims 1 to 4, or cell population according to claim 7, or obtained according to the method of any of claims 8 or 9, for use in therapy. 13. An isolated multipotent adult cell according to any of claims 1 to 4, or a cell population according to claim 7, or obtained according to the method of any of claims 8 or 9, for use in the repair and regeneration of tissues. 14. Use of an isolated multipotent adult cell according to any of claims 1 to 4, or a cell population according to claim 7, or obtained according to the method of any of claims 8 or 9, for the manufacture of a pharmaceutical composition for the repair and regeneration of tissues. 15. A method for assessingin vitro cell response to a biological or pharmacological agent, or to a combinatorial library of said agents, the method comprising: a) isolating a cell population according to claim 7, or obtained according to the method of any of claims 8 or 9, b) expanding the cell population via culturing, and c) applying a biological agent or pharmacological agent or a combinatorial library of said agents to the cell population, and assessing effects of said agents on the cultured cells.
