madrid2i

Resumen

A transformed T. lanuginosus cell is described which expresses recombinant proteins.

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CLAIMS 1. 1. A transformed T. lanuginosus cell comprising a NOI. 2. A transformed T. lanuginosus cell according to Claim 1 wherein the NOI codes for a heat stable protein. 3. A transformed T. lanuginosus cell according to Claim 1 or Claim 2 wherein the NOI is operably linked to a promoter. 4. A transformed T. lanuginosus cell according Claim 3 wherein the promoter is a fungal promoter. 5. A transformed T. lanuginosus cell according to Claim 4 wherein the promoter is derived or derivable from T. lanuginosus. 6. A method for producing a POI which comprises culturing a transformed T. lanuginosus cell comprising a NOI encoding a POI operably linked to a promoter under conditions which allow expression of the POI. 7. A method according to Claim 6 wherein the POI is secreted to the culture medium, and optionally wherein said POI is isolated and/or purified. 8. A method according to Claim 6 or Claim 7 wherein the NOI is an NOI as defined in any one of claims 2 to 7. 9. A process for transforming Thermomyces lanuginosus comprising contacting germinated spores of Thermomyces lanuginosus with lithium acetate. 10. A transformed Thermomyces lanuginosus comprising any one or more of: an NOI, the promoter having the sequence presented as SEQ ID No. 1 or a variant, derivative or fragment thereof, a trpC terminator, and the ble gene or a fragment thereof capable of imparting resistance to phleomycin.

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