Resumen
[0001] The present invention provides an expression cassette comprising at least one polynucleotide comprising a nucleotide sequence selected from the group consisting of: a) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NOs. 13-24; and b) a nucleic acid sequence that encodes a polypeptide having the amino acid sequence sharing at least 50%, preferably at least 55%, 60%, 65%, 70%, 75%, 80%, 85%, especially preferably at least 90%, 95%, 97%, 98%, 99%, or more of sequence identity with a sequence selected in the group consisting of SEQ ID NOs. 13-24. This expression cassette can be used in vivo to increase in plants the tolerance to abiotic stress, such as e.g., sulphur deficiency, nitrogen deficiency, or carbon/nitrogen imbalance. In another aspect, the invention thus relates to a transgenic plant cell comprising the expression cassette described above. [0002] La présente invention concerne une cassette d'expression comprenant au moins un polynucléotide comprenant une séquence nucléotidique choisie dans le groupe constitué de : a) une séquence nucléotidique qui code pour un polypeptide ayant la séquence d'acides aminés présentée dans 13 à 24 SEQ ID NO; et b) une séquence d'acide nucléique qui code pour un polypeptide ayant la séquence d'acides aminés partageant au moins 50 %, de préférence au moins 55 %, 60 %, 65 %, 70 %, 75 %, 80 %, 85 %, en particulier de préférence au moins 90 %, 95 %, 97 %, 98 %, 99 % ou plus d'identité de séquence avec une séquence choisie dans le groupe constitué de 13 à 24 SEQ ID NO. Cette cassette d'expression peut être utilisée in vivo afin d'accroître chez les plantes la tolérance au stress abiotique, tel que, par exemple, une carence en soufre, une carence en azote ou un déséquilibre carbone/azote. Dans un autre aspect, l'invention concerne ainsi une cellule de plante transgénique comprenant la cassette d'expression précitée.
Reivindicaciones
1. An expression cassette comprising at least one polynucleotide comprising a nucleotide sequence selected from the group consisting of: a) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NOs. 13-24;and b) a nucleic acid sequence that encodes a polypeptide having the amino acid sequence sharing at least 50%, preferably at least 55%, 60%, 65%, 70%, 75%, 80%, 85%, especially preferably at least 90%, 95%, 97%, 98%, 99%, or more of sequence identity with a sequence selected in the group consisting of SEQ ID NOs. 13-24. 2. An expression cassette according to claim 1, wherein said expression cassette has a sequence selected from the sequences represented by SEQ ID NOs. 30-41. 3. A plant cell comprising the expression cassette according to any one of claims 1 or 2. 4. A transgenic plant comprising a plant cell according to claim 3. 5. The transgenic plant of claim 4, wherein said plant is a monocot or a dicot. 6. The transgenic plant of claim 4, wherein said plant is selected from the group consisting of: maize, soybean, sunflower, sorghum, canola, wheat, alfalfa, cotton, rice, barley, millet and sugar cane. 7. A transgenic seed from the transgenic plant of claim 4. 8. A method for the production of a transgenic plant, said method comprising the transformation of a plant by an expression cassette according to any one of claims 1 or 2. 9. A method for increasing or maintaining plant yield under non-optimal conditions for sulphur, nitrogen, and/or C/N balance, said method comprising a step of growing a transgenic plant according to claim 4 under conditions of non- optimal conditions for sulphur, nitrogen, and/or C/N balance, wherein: .cndot. the yield obtained from said transgenic plant grown under said non- optimal conditions is increased as compared to the yield obtained from a plant not containing the expression cassette according to any one of claims 1 or 2, grown under said non-optimal conditions, or .cndot. the yield obtained from said transgenic plants grown under said non- optimal conditions is maintained as compared to the yield obtained from a transgenic 53 plant according to claim 4, and grown in optimal conditions for sulphur, nitrogen, and/or C/N balance 10. A method for increasing or maintaining plant yield under optimal conditions for sulphur, nitrogen, and/or C/N balance, said method comprising a step of growing a transgenic plant according to claim 4 under conditions of optimal conditions for sulphur, nitrogen, and/or C/N balance, wherein the yield obtained from said transgenic plant is increased as compared to the yield obtained from a plant not containing the expression cassette according to any one of claims 1 or 2, grown under said optimal conditions. 11. A method for selecting a plant that can be used in a breeding process for obtaining a plant with improved yield under optimal or under non optimal conditions for sulphur, nitrogen, and/or C/N balance comprising the step of selecting, in a population of plant, the plants containing the recombinant expression cassette according to any one of claims 1 or 2. 12. A method for identifying a plant with improved yield under optimal or under non optimal conditions for sulphur, nitrogen, and/or C/N balance comprising the step of identifying, in a population of plant, the plants containing the recombinant expression cassette according to any one of claims 1 or 2.
