Suggested is a process for the regio-selective manufacture of disaccharides or oligosaccharides by transglycosilation or hydrolysis of a carbohydrate donator molecule [G-R] and a glycosyl acceptor molecule [A] in the presence of a glycosidase in the presence of at least one dialkyl amide.
1. A process for the regio-selective manufacture of disaccharides or oligosaccharides by transglycosilation or hydrolysis of a carbohydrate donator molecule [G-R] and a glycosyl acceptor molecule [A] in the presence of a glycosidase in the presence of at least one dialkyl amide. 2. A process according to Claim 1, characterised in that said disaccharides or oligosaccharides are β-1,6-disaccharides or β-1,6-oligosaccharides. 3. A process according to Claims 1 and/or 2, characterised in that said carbohydrate donor molecules [D] follow formula (I) [G]-[R] (I) in which [G] represents a monosaccharide, disaccharide or oligosaccharide moiety having an anomeric carbon and [R] stands for a leaving group attached to said anomeric carbon. 4. A process according to any of the preceding Claims 1 to 3, characterised in that said carbohydrate donor molecules [G-R] include as the leaving group [R] hydrogen, fluor, a nitrophenyl group, a monosaccharide, a polysaccharide or a thioglycoside. 5. A process according to any of the preceding Claims 1 to 4, characterised in that said glycosyl acceptor molecules [A] represent monosaccharides, disaccharides or oligosaccharides, or a fatty alcohol, optionally substituted by an ester or amide group. 6. A process according to any of the preceding Claims 1 to 5, characterised in that said glycosyl acceptor molecule [A] represent N-acetyl-D glucosamine or N-acetyl-D glucosamine glycoside. 7. A process according to any of the preceding Claims 1 to 6, characterised in that said enzyme is a β-galactosidase. 8. A process according to any of the preceding Claims 1 to 7, characterised in that said dialkyl amides follow formula (IV) R<1>CO-NR<2>R<3> (IV) in which R<1>CO stands for a linear or branched, saturated or unsaturated, optionally hydroxysubstituted radical having 1 to 21 carbon atoms, and R<2> and R<3> independently from each other represent alkyl radicals having 1 to 6 carbon atoms. 9. A process according to Claim 7, characterised in that said dialkyl amides are dimethylamides based on C<6>-C<10> fatty acid, C<8>-C<10> fatty acid, lactic acid or their mixtures. 10. A process for the production of carbohydrates, characterised in that carbohydrate donor molecules [G-R] according to any of the preceding Claims 1 to 9 are subjected to hydrolysis in the presence of an enzyme capable to hydrolyse the bond between the saccharide moiety [G] and the leaving group [R], and at least one dialkyl amide.