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METHOD OF INCREASING OR INHIBITING GENE EXPRESSION BY MAKING USE OF PROMOTERS OF GLUTAMATE DEHYDROGENASE, {BETA}-N-HEXOSOANIMIDASE GENE AND GAMMA-ACTIN PROMOTERS AND USE OF SUCH PROMOTERS FOR PREPARING ANTIBIOTICSCM Patents

The invention relates to promoters of the genes glutamate desydrogenase, .beta.-acetylhexosaminidase and .gamma.-actine and their use in systems of expression, secretion and anti-sens of filamentary funghi. The invention also relates to the use of the promoters of the genes which code: (I) glutamate desydrogenase NADP depending (EC.1.4.1.4) of Penicillium chrysogenum, (II) .gamma.-N-actylhexosaminidase (EC.3.2.1.52) of Penicillium chrysogenum and (III) .gamma.-actine of Penicillium chrysogenum and Acrimonium chrysogenum, which can be used for the construction of potent vectors of expression and secretion useful both for P. chrysogenum and for A. chrysogenum and related species. Said promoters can also be used for blocking the genic expression through anti-sens construction. Under the control of the above mentioned promoters, it is possible to conduct the expression of other genes in filamentary fungi, thereby increasing the production of antibiotics and/or proteins inherent to the same.

Datos clave

CategoríaInvención

Índice de la ficha

Updated at
05/11/2026
Numero publicacion
WO.9839459.A1
Fecha publicacion
11/09/1998
Anio publicacion
1998

En detalle

Reivindicaciones

35. Transformed non-human organisms characterized in that the DNA sequences of Claims 8 to 16, totally or partially included in the vectors of Claims 21 to 26, obtainable by the process described in Claims 27 to 34, have been introduced into them. - 60 - 36. - Transformed organism according to Claim 35, characterized by consisting of a prokaryote, preferably Escherichia coli or an actinomycete. 37. - Transformed organism according to Claim 35, characterized by consisting of a eukaryote, preferably of the genus Penicillium, Aspergillus, Acremonium or Saccharomyces. 38. - Transformed organism according to Claim 36, characterized by consisting preferably of Penicillium chrysogenum, Aspergillus nidulans, Acremonium chrysogenum or Saccharomyces cerevisiae. 39. - Organism according to Claim 36, characterized by consisting of pure strains represented by CECT4849, CECT4852, CECT4851 and CECT4850 or mutants and transformed derivatives thereof. 40. - Use of the transformed organisms of Claims 35 to 39, in the production of antibiotics. 41. - Use of the transformed organisms of Claims 35 to 39, in the production of penicillin. 42. - Use of the transformed organisms of Claims 35 to 39, in the production of cephalosporins.

Etiquetas

Inventores
Fuente Jose Luis BarredoJose Luis Barredo FuenteMarta Rodriguez SaizSaiz Marta RodriguezValle Miguel Angel MorenoMiguel Angel Moreno ValleVieja Alfonso J Collados de laAlfonso J. Collados de la ViejaFrancisco Salto MaldonadoMaldonado Francisco SaltoGarcia Bruno DiezBruno Diez GarciaMoreno Valle Miguel AngelMoreno Valle, Miguel AngelDiez Garcia BrunoDiez Garcia, BrunoRodriguez Saiz MartaRodriguez Saiz, MartaBarredo Fuente, Jose LuisBarredo Fuente Jose LuisSalto Maldonado FranciscoSalto Maldonado, FranciscoCollados de la Vieja Alfonso JCollados de la Vieja, Alfonso J.Jos Luis Barredo FuenteFuente Jos Luis BarredoMarta Rodrigues SaizSaiz Marta RodriguesMoreno Miguel AngelMiguel Angel MorenoBarredo Fuente José LuisCollados de la Vieja Alfonso J.Jos� Luis Barredo FuenteAlfonso J Collados de la ViejaCollados de la Vieja, Alfonso Juan바레도 푸엔떼,호세 루이스로드리게즈 사이즈, 마르따모레노 발레, 미구엘 앙헬꼴라도스 데라 비에하, 알폰소 제이살토 말도나도, 프란시스코디에즈 가르시아, 브루노Barredo-Fuente Jose LuisBarredo-Fuente,Jose LuisRodriguez-Saiz MartaRodriguez-Saiz,MartaMoreno-Valle Miguel AngelMoreno-Valle,Miguel AngelCollados-De-La-Vieja Alfonso JCollados-De-La-Vieja,Alfonso J.Salto-Maldonado,FranciscoSalto-Maldonado FranciscoDiez-Garcia,BrunoDiez-Garcia BrunoBaredo Fuente Jose LuisBARREDO Fuente,Jose LuisRODRIGUEZ Saiz,MartaMORENO Valle,Miguel AngelJ Collados de la Vieja AlfonsoCollados de la Vieja,Alfonso J.SALTO Maldonado,FranciscoDiez Garcia,BrunoBarredo Fuente J LBarredo Fuente J. L.Rodriguez Saiz MRodriguez Saiz M.Moreno Valle M AMoreno Valle M. A.Salto Maldonano F.Salto Maldonano FDiez Garcia BDiez Garcia B.Moreno Valle Migeul Angel
Solicitantes
Antibioticos S.A.U.Ticos S.A.Antibioticos, SAU, Madrid안티비오티코스,에스.에이.유Antiobiotics S.A.Antibiotics S.A.U.
Clasificacion ipc
C12N 1/ 15 A IC12N 15/ 00 A IC12N 15/ 09 A IC12N 15/ 113 A IC12N 15/ 53 A IC12N 15/ 62 A IC12N 15/ 70 A IC12N 15/ 80 A IC12N 9/ 06 A IC12N 9/ 24 A IC12P 35/ 00 A IC12P 37/ 00 A I
Clasificacion cpc
4B024/AA034B024/AA204B024/BA674B024/BA804B024/CA024B024/CA204B024/DA064B024/DA114B024/DA124B024/EA044B024/FA024B024/FA104B024/GA114B024/GA194B024/GA214B024/HA204B050/CC034B050/CC054B050/DD034B050/LL014B050/LL054B064/AH124B064/AH164B064/CA024B064/CA034B064/CA054B064/CA064B064/CA194B064/CC244B064/DA024B065/AA264B065/AA584B065/AA604B065/AA684B065/AB014B065/AC154B065/BA024B065/CA244B065/CA284B065/CA31C12N1/15C12N15/00C12N15/00&AC12N9/06&BC12N9/24C12P35/00C12P37/00C12R1/645C12R1/66C12R1/82435/252.3435/252.33435/254.11435/254.21435/254.3435/254.5435/320.1435/471435/483435/484435/69.1530/350536/23.74536/24.1536/24.5435/243435/254.1435/41435/455435/475435/476435/71.1530/371536/22.1536/23.1536/25.3
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